WBC and associated changes Flashcards
absolute WBC count
WBC * % cell type
quantitative measure
relative WBC count
% cell type
depends on number of other line
granulocyte
neutrophil
eosinophil
basophil
myeloperoxidase
neutrophil primary granule
alkaline phosphatase
neutrophil secondary granule
leukemoid reaction
elevated WBC with immature cells
20-50k neutrophils
indicates stress
Dohle bodies present
leukoerythroblastic blood picture
leukemoid WBC + elevated RBC
reactive, neoplastic, marrow disorder
neutrophilia
elevated neutrophils bacterial infection tissue necrosis physiologic stress hematologic disorders drug reaction metabolic
normal WBC count
5-10k
neutropenia
decreased neutrophils
causes of decreased marrow production of neutrophils
toxicity aplastic anemia myelophthisis nutritional deficiency collagen diseases
causes of increased destruction/use of neutrophils
splenic sequestration
viral infections
immune mediated
prolonged inflammation
drugs associated with neutropenia
antibiotics
monocyte main function
phagocytosis
main cause of monocytosis
neutropenia
chronic inflammation
malignancy
eosinophil function
limit chronic inflammatory responses
eosinophil graunles
peroxidase
acid phosphatase
eosinophil membrane receptors
IgG
complement
causes of eosinophilia
parasite infection
allergy
drug reactions
chronic hypereosinophilic syndrome
persistent elevation in eosinophils
rare
needs to be treated early
basophil granules
histamine
heparin-like mucopolysaccharides
basophil membrane receptors
IgE
basophil function
hypersensitivity reactions
basophilia cause
myeloproliferative disorders- CML
hypersensitivity reactions
which is more b or t cells
T cells
which lives longer b or t cells
T cells
b cell function
humoral immunity
antibody production
T cell function
cellular immunity
b cell inducer
mechanical interference with lymphocytes
thoracic duct obstruction
intestinal lymphatics with poor absorption
layers of the folicle
germinal center- CD10+
mantle- CD5+
marginal zone- CD5/10-
precursor B lymphoblasts
precursor for entire b cell lineage
TdT
naive mature B cell
circulating in blood
primary follicle and mantle zone
sIg- M D
have not found antigen
germinal center blasts- b cells
found antigen, returned to germinal center secondary follicle
CD10, BCL6 +
BCL2 and sIg negative
IgM switch to IgG or IgA
CD10+
germinal center lymphocyte
found the antigen pair
BCL2
anti apoptosis
in all cells except centroblasts
germinal center centrocytes
secondary germinal center resting cells
sIg
BCL2 +
IgM or IgA
plasma cells
return to bone marrow
CD79a, CD138 +
BCL6, sIg, CD20, CD5, CD10 -
produces antibodies
memory B cells
go back to lymphoid organ origin
sIg, CD20, BCL2 +
BCL6, CD5, CD10 -
IgM
lymphocytosis of acute infections
infectious mono (EBV)
pertussis
other viruses
lymphocytosis
chronic infection
thyrotoxicosis
drug sensitivity
hematologic malignancy
lymphopenia
immunodeficiency syndromes
lymphocyte destruction
mechanical loss
neoplasms
monocyte granules
lysozyme
myeloid maturation
myeloblast
promyelocyte
myelocyte
metamyelocyte
CD20
memory b cell marker
CD138, CD79a
plasma cell marker
