w7 safe blood collection Flashcards
are bacteria present in blood
yes but usually they are quickly eliminated by white blood cells, liver and spleen.
-bacteremia, septicaemia develop when infection occurs from multiplication of bacteria in blood stream faster than they can be eliminated
when are blood cultures ordered
-to aid in diagnosis when there is a fever of unknown origin FUO or pyrexia of unknown origin PUO
what do blood cultures help to determine
- presence and extent of infection
- identity of organisms
- antibiotic to which bacteria will be susceptible
when to collect cultures
- just before anticipated fever spike
- immediately following a fever spike
- before antibiotic therapy
- from more than one site
collection set of blood vials
one aerobic and one anaerobic O2 and AnO2. to detect all types of bacteria present.
-ordered at intervals 2x or 3x to increase the chance of isolation- collected from second collection sites
consecutive collection technique
collect both vials from one arm and then the other arm right away -indicate on bottle which arm was used RA or LA- common with patients experiencing acute conditions where administration of antibiotics are immediately required
Time interval technique
collect the second site at a given time following the collection of the first set. 20 to 30 minutes are usual time intervals
limitations to vials
it is a large volume
10ml taken from each vail, 4 vials taken total 40ml of blood taken
where is blood culture taken
performed at bedside
what volume are blood vials filled to
10ml mark down on bottle, they will fill more than that - for adults
kid volumes based on weight
what to remember
clean with alcohol as usual
clean for 30 seconds with iodine then let dry
dont repalpate unless you clean your finger with iodine and dont touch anything
clean tops of vials and leave alcohol swabs on
in total 3 alcohol swabs, 1 iodine swab
what to do if insufficient volume
note it on req
split between the two vials if more than 2ml
if less that two ml inoculate aerobic vial only
do not inoculate paediatric vial in place of adult vial
what to clean with for blood culture
70% isopropyl alcohol
povidone iodine then chlorhexidine gluconate do a friction scrub for 30 seconds then allow t0 dry for one to two minutes
-use gauze after because the iodine is sticky
what is blood culture done with
butterfly need
*since air is present in tube use aerobic one first
order of draw
aerobic, anaerobic, blood tubes in order of draw
what to use for blood culture with bad veins
syringe is okay to use, 20ml syringe would be used for fill both - its not recommended because it could contaminate vials.
make sure to use safety transfer device.