Virology

Question 1

Detection virus specific antibodies in serum

Answer 1

ELISA enzyme linked immunosorbent assay

Question 2

Direct demonstration virus/ part virus

Answer 2

virus visualisation via electron microscopy
viral antigen immunofluorescence IF, ELISA
viral nucleic acid - PCR

Question 3

Demonstration of effect of virus

Answer 3

virus isolation = growth of virus in cell culture

Question 4

IgM vs IgG

Answer 4

IgM - first Ab to appear followed by much higher titre of IgG
IgM can raised in primary and re-infection
IgG avidity testing
IgG cross placenta while IgM does NOT
persists 6-9 months

Question 5

Seroconversion

Answer 5

IgG negative to positive

Question 6

Testing viruses

Answer 6

Measles, Rubella, Parvovirus B19, Mumps - blood only
Rabies - saliva
Polio - stools
SSPE - CSF
Respiratory infxn - NPA SPUTUM NOT RECOMMENDED
Hepatitis A,B,C = blood
Rotavirus - stool

Question 7

HIV-DNA PCR Specimens

Answer 7

EDTA blood

Dried Blood Spot cards