Video: 15.2 cAMP Signaling

Question 1

What is Adenylyl Cyclase protein?

Answer 1

An membrane-bound enzyme whos catalytic domain is activated by activated alpha subunits (GTP bound Subunit)

Question 2

Activated Adenylyl Cyclase does what?

Answer 2

Converts ATP to cAMP

Question 3

What does cAMP do?

Answer 3

Relays signal from G-protein coupled receptor to components inside the cell

Question 4

What’s the interaction between cAMP and PKA?

Answer 4

cAMP binds to PKA regulatory subunits, activating PKA

Question 5

What is the makeup of an inactive PKA?

Answer 5

2 Regulatory subunits, 2 Catalytic subunits

Question 6

What is the makeup of an active PKA?

Answer 6

cAMP bound to both Regulatory subunits
Activated Catalytic subunits that separate from Regulatory subunits

Question 7

Binding of cAMP to Regulatory subunits wil…

Answer 7

activate Catalytic subunits which will phosphorylate target proteins.

Question 8

What do activated catalytic PKA subunits do?

Answer 8

phosphorylate target proteins

Question 9

What do activated catalytic PKA subunits do in endocrine cells?

Answer 9

enter the nucleus, and phosphorylates CREB

Question 10

What happens when catalytic PKA subunits enter the nucleus?

Answer 10

subunit phosphorylates CREB causing ATP to become ADP, attracting a CREB-binding protein

Question 11

What does the CREB binding protein do?

Answer 11

Binds to phosphorylated CREB and binds to promotor on regulatory region of gene