Unit 3: Membrane structure and fluidity Flashcards
What protein are associated with the cell/plasma membrane ONLY
transmembrane
integral
peripheral
lipid anchored protein
how does the integral protein interact with the lipid bilayer/ cell membrane
goes into the membrane and comes out on the same side
how does the transmembrane protein interact with the lipid bilayer/ cell membrane
going into the membrane and out the OTHER side
how does the peripheral protein interact with the lipid bilayer/ cell membrane
weak bonds with the protein that’s bonded with the membrane
how does the anchored protein interact with the lipid bilayer/ cell membrane
anchored to the phosphate on the membrane
what does a carbohydrate/sugar make on the membrane and what does it do
glycoprotein and glycolipid
branch
what is a glycoprotein made from and what does it attach to
oligosaccharide + protein
what is a glycolipid made from and what does it attach to
oligosaccharide + lipid
what do oligosaccharides do
cell identification and cell protection
asymmetric membrane
both sides of the phospholipid are different from each other
salt affects which protein
peripheral because it bonds with it and it breaks from the other protein
detergent affects which proteins
transmembrane, integral and lipid anchored
where are the oligosaccharides and what do they look like
outside the cell and like hair
membrane fluidity experiments
FRAP and Hybridoma
what happened in the FRAP experiment
a cell was coated in a fluorescent color and a part of the cell was bleached. Waited to see if the bleach separated
what happened in Hybridoma experiment
part of a human cell (colored) and part of a mouse cell (colored) and waited to see if the color mingled together
Membrane fluidity def
area of movement protein and lipid has within cell
what happens to fluidity in relation to temp
temp up, fluidity increase
temp down, fluidity decreases
how do bacteria and plants regulate fluidity
chain/tail length
cis unsaturated/ saturated
how do animals regulate fluidity
cholesterol “fluidity buffer”
regulate internal temp
amphipathic
having both hydrophobic and hydrophilic
how does cholesterol buffer fluidity
hot: bind lipid tops, so it doesn’t move around as much
cold: keeps lipids apart
detergent has what shape and charge
head of a lipid and a tail, head-polar, tail-non-polar
fluidity deals with what part of the phospholipid
non polar tails
