Unit 3: Membrane structure and fluidity

Question 1

What protein are associated with the cell/plasma membrane ONLY

Answer 1

transmembrane
integral
peripheral
lipid anchored protein

Question 2

how does the integral protein interact with the lipid bilayer/ cell membrane

Answer 2

goes into the membrane and comes out on the same side

Question 3

how does the transmembrane protein interact with the lipid bilayer/ cell membrane

Answer 3

going into the membrane and out the OTHER side

Question 4

how does the peripheral protein interact with the lipid bilayer/ cell membrane

Answer 4

weak bonds with the protein that’s bonded with the membrane

Question 5

how does the anchored protein interact with the lipid bilayer/ cell membrane

Answer 5

anchored to the phosphate on the membrane

Question 6

what does a carbohydrate/sugar make on the membrane and what does it do

Answer 6

glycoprotein and glycolipid

branch

Question 7

what is a glycoprotein made from and what does it attach to

Answer 7

oligosaccharide + protein

Question 8

what is a glycolipid made from and what does it attach to

Answer 8

oligosaccharide + lipid

Question 9

what do oligosaccharides do

Answer 9

cell identification and cell protection

Question 10

asymmetric membrane

Answer 10

both sides of the phospholipid are different from each other

Question 11

salt affects which protein

Answer 11

peripheral because it bonds with it and it breaks from the other protein

Question 12

detergent affects which proteins

Answer 12

transmembrane, integral and lipid anchored

Question 13

where are the oligosaccharides and what do they look like

Answer 13

outside the cell and like hair

Question 14

membrane fluidity experiments

Answer 14

FRAP and Hybridoma

Question 15

what happened in the FRAP experiment

Answer 15

a cell was coated in a fluorescent color and a part of the cell was bleached. Waited to see if the bleach separated

Question 16

what happened in Hybridoma experiment

Answer 16

part of a human cell (colored) and part of a mouse cell (colored) and waited to see if the color mingled together

Question 17

Membrane fluidity def

Answer 17

area of movement protein and lipid has within cell

Question 18

what happens to fluidity in relation to temp

Answer 18

temp up, fluidity increase

temp down, fluidity decreases

Question 19

how do bacteria and plants regulate fluidity

Answer 19

chain/tail length

cis unsaturated/ saturated

Question 20

how do animals regulate fluidity

Answer 20

cholesterol “fluidity buffer”

regulate internal temp

Question 21

amphipathic

Answer 21

having both hydrophobic and hydrophilic

Question 22

how does cholesterol buffer fluidity

Answer 22

hot: bind lipid tops, so it doesn’t move around as much
cold: keeps lipids apart

Question 23

detergent has what shape and charge

Answer 23

head of a lipid and a tail, head-polar, tail-non-polar

Question 24

fluidity deals with what part of the phospholipid

Answer 24

non polar tails