TRIMMING AND SECTIONING Flashcards
Process of REMOVING EXCESS WAX after embedding
TRIMMING
Tissue should be surrounded by at least _____ of wax
2 mm of wax
Side, tips, and bottom of the tissue are trimmed using a KNIFE or a blade to form a truncated pyramid or FOUR-SIDED PRISM
COARSE TRIMMING
Block is placed in the MICROTOME setting the adjuster at 15 mm or by advancing the block using the coarse feed mechanism where the surface is trimmed away until the desire tissue surface has been exposed
FINE TRIMMING
Also known as CUTTING; process wherein a processed tissue is cut into uniformly thin slices or sections using microtome to facilitate studies under the microscope
SECTIONING
SECTION CUT IS PICKED BY:
- Index finger
- Camel hairbrush
- Spatula
- Flat-bladed forceps
For routine histologic procedures, tissue sections are commonly cut between _________ in thickness
4 to 6 MICRA in thickness
Section thickness
PARAFFIN SECTIONS -
CELLOIDIN SECTIONS -
Frozen sections -
Ultrathin sections -
Section thickness
PARAFFIN SECTIONS - 4 to 6 micra
CELLOIDIN SECTIONS - 10 to 15 micra
Frozen sections - 10 micra
Ultrathin sections -
- 0.5 to 1 micron for semi-thin sections
- 500 to 1200 angstrom or 50-120 nm for ultrathin sections
To remove wrinkles, faults and to FLATTEN THE RIBBONS, it must be placed to the _______________
FLOTATION WATER BATH at 45-50C (6-10C BELOW the melting point of wax)
Approximately _________ should be long enough for a ribbon to flatten
30 seconds
______ is the removal of ribbons from the flotation water bath
FISHING-OUT/FLOATING-OUT
These are squamous cell contamination that result from the technician placing UNGLOVED fingers to the water bath
FLOATERS
Drying of ribbons may be done using:
Incubator
Paraffin oven
Hot plate
