MOUNTING Flashcards
Process of PUTTING COVERSLIP on the stained tissue using mounting medium
Mounting
To avoid distortion of the image, the REFRACTIVE INDEX of the mountant should be as near as possible to that of the glass which is _______.
1.518
Components of mounting medium:
- Gelatin, Glycerin jelly and Gum Arabic - to solidify the medium
- Glycerol - To prevent cracking and drying of the preparation
- Sugar - To raise/increase the refractive index
Aqueous mounting media:
- Water
- Apathy’s medium (Refractive index 1.52)
- Glycerin Jelly (Refractive index 1.47)
- Farrant’s Medium (Refractive index 1.43)
- Brun’s fluid
“WAGFB”/ Basa ang cp mo, WAG magFB”
Mounting media for preparations that have been cleared in xylene or toluene
Resinous Mounting media
Natural resin extracted from a CANADIAN TREE; MOST COMMONLY USED MOUNTING MEDIUM
CANADA BALSAM
Canada Balsam has a refractive index of:
1.524
Canada balsam is a natural resin extracted from a canadian tree known as:
Abus balsamea
Process of SEALING THE MARGINS of the coverlsip:
RINGING
_______ which is made up of 2 parts of paraffin wax and 4 to 9 parts of powdered resin is the MOST COMMONLY USED RINGING MEDIUM
KRONIG CEMENT
_________________ is an UNCOMMON tissue cutting artifact that leads to the formation of strips of tissue separated by narrow clear spaces.
“VENETIAN BLINDS”
What causes venetian blinds phenomenon?
caused by NICKED BLADES