EQUIPMENTS USED IN HISTOTECHNOLOGY/TISSUE PROCESSING

Question 1

Temperature-dependent equipments in histopath:

Answer 1

Tissue processer: 40-42C
Flotation water bath: 45-50C
Paraffin embedding center: 55-60C

Question 2

Automatic tissue processer automatically ___, _____, ____ and _____ tissues

Answer 2

Fixes, Dehydrates, Clears, and Infiltrates tissues

Question 3

Automatic tissue processor processes tissue for about ______ hours

Answer 3

16 hours

Question 4

There are _____________ individual processing steps with a ______ 1-liter capacity glass beakers and ____ thermostatically controlled wax baths

Answer 4

TWELVE (12) INDIVIDUAL PROCESSING STEPS with a TEN (10) 1-liter capacity glass beakers and TWO (2) thermostatically controlled wax baths

Question 5

Wax bath thermostat should be at least _____ above the melting point of the wax

Answer 5

Wax bath thermostat should be at least 3C above the melting point of the wax

Question 6

TWO MAIN TYPES OF PROCESSORS:

Answer 6

1. Tissue-transfer (or “dip and dunk”) - specimens are transferred from container to container
2. Fluid transfer (or “enclosed”) - specimens are held in a single process chamber or retort and fluids are pumped in and out as required

Question 7

In automatic tissue processors, the _______ of the tissue into reagent containers provided ______ needed for the processing of the tissue

Answer 7

In automatic tissue processors, the VERTICAL OSCILLATION of the tissue into reagent containers provided AGITATION needed for the processing of the tissue

Question 8

_________ is a part of an automatic tissue processor responsible for controlling the TIME necessary for each processing step

Answer 8

ELECTRICAL CLOCK

Question 9

REMEMBER!

VACUUM EMBEDDING gives the FASTES RESULT (5-15 minutes)

Question 10

Primary function of flotation water bath:

Answer 10

FLATTEN THE RIBBONS

Question 11

FLOTATION WATER BATH is set at a temperature of _______ approximately ___________ to melting point of the wax used

Answer 11

FLOTATION WATER BATH is set at a temperature of 45-50C approximately 6-10C lower than to melting point of the wax used

Question 12

Infiltration/Embedding process is regulated at temperature:

Answer 12

55-60C

Question 13

Drying process - done at ______ for two hours

Answer 13

56 to 60C for two hours

Question 14

Used in handling sections during cutting, and for removing folds and creases on the sections during “floating out” in water bath

Answer 14

FORCEPS and SQUIRREL HAIR BRUSH

Question 15

Preferred slide dimensions for routine work:

Answer 15

76x25 mm slides that are
1 to 1.2 mm thick are preferred

refractive index of 1.518

Question 16

Slotted jar for holding from 5 to 9 slides

Answer 16

Coplin jars

Question 17

staining equipment holding from 5 to 19 slides:

Answer 17

Slotted staining dishes

Question 18

staining equipment holding from 10 to 30 slides:

Answer 18

Metal or glass staining racks or carriers