translation Flashcards
what shape is the tRNA molecule
a clover, bottom third contains the anticodon
what is an anticodon
sequence of 3 nucleotides found on tRNA contains complementary base pairs to mRNA
rough ER link to protein
where proteins are synthesised
-flattened sacs and tubules encrusted with ribosomes
-proteins that are secreted by the cell are synthesised through the rough ER
-passed onto the golgi body
where are proteins synthesised if they are staying inside the cell
-in the cytosol (liquid of cytoplasm)
what is the Golgi apparatus’ link to protein
packages and modified proteins
-flattened fluid sacs
-vesicles containing newly synthesised proteins fuse with Golgi apparatus
-the Golgi apparatus processes the protein
-vesicles containing finished products are pinched off the Golgi body
-discharged out of cell by exocytosis
steps of translation
-the mRNA has left nucleus via the nuclear pore and entered cytoplasm
-the mRNA binds to the small subunit of a ribosome at its start codon
-a tRNA molecule with a complementary anticodon binds to the start codon on the mRNA bringing the first AA
-the ribosome reads the next codon on the mRNA and a second molecule binds to the codon with the anticodon UGC, brining the second amino acid for polypeptide chain
-peptide bonds form between first 2 amino acids
-ribosome moves down mRNA and first tRNa is released, ribosome reads next codon and complementary tRNA molecule brings an amino acid
-process continues to form a polypeptide chain
-the stop codon tells ribosome when to stop translating mRNA
-the polypeptide chain coils to form a protein
what is the start codon of translation
AUG
what is always the first amino acid
-methionine
what is the second codon of translation
-UGC
what is always the second amino aicd
-threonine
how many tRNA molecules can bind to a mRNA molecule at the same time
-only 2
how do peptide bonds form between the amino acids
-in a condensation reaction which is catalysed by peptidyl transferase
what is peptidyl transferase
-an RNA component of a ribosome
what are the subunits of ribosome composed of
-protein
-form of RNA called rRNA (peptidyl transferase)