factors effecting enzyme controlled reactionbs

Question 1

how do u measure initial rate of reaction

Answer 1

-using a tangent drawn where the line starts to increase

Question 2

at what point do scientists measure the rate of reaction

Answer 2

-at the start
-before any factors have had time to change

Question 3

In a graph of amount of substrate against rate of reaction, what is the limiting factor up until the plateau

Answer 3

-substrate concentration
-after, sc is no longer limiting factor because all enzymes present are working at maximum rate

Question 4

what is directly proportional on a rate of reaction/amount of substrate graph and why

Answer 4

-amount of substrate added and rate of reaction
-for every substrate you add, there are enzymes for it to bind to

Question 5

why is there an extreme but equal drop either side of optimum on a rate of reaction/ pH graph

Answer 5

-reducing or increasing pH away from optimum reduces rate of reaction
-because concentration of H+ in solution affects tertiary structure of the enzyme molecule

Question 6

why does increasing the temp beyond the optimum reduce the rate of reaction

Answer 6

-it breaks the bonds that holds enzymes tertiary structure in place