The Medical Microbiologist

Question 1

3 roles of medical microbiology lab:

Answer 1

isolate and identify microorganisms in a clinical specimen
determines susceptibilty of isolates to antimicrobial agents
provide medical doctors with information about presence or absence of microorganisms whichare thought to be capable of spreadnig infectious disease.

Question 2

Medical microbiology lab receives specimens from (5)

Answer 2

urinary tract infection
respiratory tract infection
gastrointestinal tract infection
STD infections
wound/tissue infection

Question 3

to be useful in diagnosis and treatment, specimens must be (6)

Answer 3

In adequate supply
of high purity
relevant
collected carefully
transported quickly
ideally obtained before microbial therapy starts

Question 4

specimens cant be collected using: (5)

Answer 4

sterile swabs
sterile container
intubation
needle aspiration
catheter

Question 5

why must organisms be transported rapidly?

Answer 5

organisms may grow in sputum or urine samples and give misleading results,
in specimens such as throat swaps, organisms may survive poorly

Question 6

4 transport medias for specimens

Answer 6

for fluids and tissues none is required
swabs should be transport in medium that keeps them alive but does not stimulate growth
agar and charcoal
suspected anaerobic sample should be injected into anaerobic vial with indicator medium

Question 7

initial identification of bacteria is found by`(4)

Answer 7

source of specimen
microscopic appearence and gram stain
pattern of growth on selective, differential or enrichment media
haemolytic, metabolic and fermentative properties discovered

Question 8

3 simple biochemical tests on specimen

Answer 8

catalase, oxidase, coagulase

Question 9

MALDI-TOF specimen identification:
can generate results in ….
can identify to … level and sometimes … level
MALDI-TOF stands for

Answer 9

minutes
species
strain
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

Question 10

Bacteriophage typing is based on

Answer 10

specificty of phage surface receptors for bacterial cell surface receptors