The Effect Of PH on Enzyme Activity

Question 1

Procedure

Answer 1

Put a drop of iodine solution into every well in the spotting tile

Place a Bunsen burner on a heat proof mat and a tripod and gauze over the bunsen burner

Place a beaker of water over the flame until it is around 35 degrees using a thermometer .

Use a syringe to add 1 cm3 of amylase solution and 1cm3 of buffer solution with a PH of 5 to a boiling tube . Place the tube into the hot water

Use a different syringe to add 5 cm3 of starch solution to the boiling tube

Mix the contents of the tube and start a stop clock

Take a fresh sample of the water every 30 seconds and put a drop of it onto the well of the spotting tile

When the Iodine remains a browny orange the starch is not present

Repeat the experiment with buffer solutions of a different PH value

Question 2

How do you calculate rate of reaction

Answer 2

1000/ time