tests for food Flashcards
How to prepare food sample
Break food up using mortar and pestle
Put into beaker with distilled water
Stir with glass rod until some dissolve
Filter some with the funnel to get rid of solid bits of food
What types of Sugars are there?
Non reducing and Reducing
What type of Sugar does Benedicts test test for?
Reucing sugars
Benedicts test?
Transfer 5cm3 of food sample to test tube
Prepare water bath at 75C’
Add 10 drops of benedicts solution to test tube and place in water bath for 5 minutres
Use test tube to put in water bath
Blue –> Green Yellow or Brick red depending on Concentration
Test for Starch?
Iodine Test
5cm3 of food sample and add few drops of Iodine Solution
Shake gently
Browny Orange colour –> Blue Black
Test for Proteins?
Biuret Test
2CM3 OF SAMPLE TO TEST TUBE
2CM3 OF BIURET SOLUTION (POTASSIUM HYDROXIDE AND COPPER SULFATE) TO SAMPLE
TURNS IT BLUE
GENTLY SHAKE
BLUE–>PINK OR PURPLE
Test for Lipids?
Add FOOD SAMPLE TO TEST TUBE
Add 2cm3 of Ethanol
Shake Vigorously
Add 2cm3 of water
Cloudy White colour
What are examples of Polysacharrides?
Starch Cellulose and Glycogen
What two things are triglecyrides made from?
Glycerol and Three Fatty Acids
What are the problems with increasing temperature to speed up the rate of reaction?
requires a lot of energy
can damage cells
speeds up non-useful reaction
Definition of a Catalyst?
A substance which increases the rat of reaction by using an alternative pathway without being changed or used up
Enzymes?
Biological Catalysts