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HISTOPATH > STAINING LAB (2) > Flashcards

STAINING LAB (2) Flashcards

1
Q

process of applying dyes on the sections to see and study the
architectural pattern of the tissue and physical characteristics of the
cells

A

Staining

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2
Q

tissues and cells display varying affinities for most dyes and stains used during the process

A

Staining

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3
Q

Has greater affinity for basic dyes

A

Acidic Structure

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4
Q

Has greater affinity for acidic dyes

A

Basic structures

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5
Q

a related procedure that makes use of heavy metal salts that are selectively precipitated on certain cellular and tissue components

A

Impregnation

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6
Q

used for silver staining of nervous tissue and demonstration of reticulin

A

Impregnation

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7
Q

What is the most commonly used reagent in impregnation?

A

Silver nitrate

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8
Q

What are the three major types of Staining?

A
  1. HISTOLOGICAL STAINING
  2. HISTOCHEMICAL STAINING/HISTOCHEMISTRY
  3. IMMUNOHISTOCHEMICAL STAINING
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9
Q

tissue constituents are demonstrated in sections by direct interaction with a dye or staining solution

A

HISTOLOGICAL STAINING

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10
Q

producing coloration of the active tissue component

A

HISTOLOGICAL STAINING

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11
Q

employed to demonstrate the general relationship of tissues and cells with differentiation of nucleus and cytoplasm

A

HISTOLOGICAL STAINING

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12
Q

Examples: microanatomic stains, bacterial stains and specific tissue stains
(muscles, CT, and neurologic stains)

A

HISTOLOGICAL STAINING

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13
Q

tissue constituents are studied through chemical reactions that will permit microscopic localization of a specific tissue substance

A

HISTOCHEMICAL STAINING/HISTOCHEMISTRY

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14
Q

Examples: Perl’s Prussian blue reaction for hemoglobin, Periodic Acid Schiff staining for carbohydrates

A

HISTOCHEMICAL STAINING/HISTOCHEMISTRY

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15
Q

active reagent serves as the substrate upon which the enzymes act

A

Enzyme histochemistry

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16
Q

final opacity of coloration produced from the substrate rather than the tissue

A

Enzyme histochemistry

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17
Q

combination of immunologic and histochemical techniques that allow phenotypic markers to be detected and demonstrated

A

IMMUNOHISTOCHEMICAL STAINING

18
Q

what are the labels that immunohistochemical staining is using

A

monoclonal/polyclonal, fluorescent-labeled, enzyme-labeled antibodies

19
Q

What are the methods of staining?

A

DIRECT STAINING
INDIRECT STAINING
PROGRESSIVE STAINING
REGRESSIVE STAINING
METACHROMATIC STAINING
COUNTERSTAINING
METALLIC IMPREGNATION
VITAL STAINING

20
Q

process of giving color to the sections by using aqueous or alcoholic dyes

A

DIRECT STAINING

21
Q

process whereby action of dye is intensified by adding another reagent which serves as a link/bridge between tissue and dye making staining reaction possible

A

INDIRECT STAINING

22
Q

serves as a link/bridge between tissue and dye making staining reaction possible

A

(MORDANT)

23
Q

may be applied to tissue before staining or may be included in the staining process, or may be incorporated as part of the dye solution itself

A

Mordant

24
Q

not essential to the chemical union of tissue and dye; does not participate in the staining reaction, but merely accelerates or hastens the speed of staining reaction by increasing the staining power and selectivity of the dye

A

ACCENTUATOR

25
Q

process whereby tissue elements are stained in a definite sequence, and the staining solution is applied for specific periods of time or until the desired intensity of coloring of the different tissue elements is attained

A

PROGRESSIVE STAINING

26
Q

no decolorization or washing after the application of the dye

A

PROGRESSIVE STAINING

27
Q

tissue is first over stained to obliterate cellular details, and excess stain is removed or decolorized from unwanted parts of the tissue, until the desired intensity of color is obtained

A

REGRESSIVE STAINING

28
Q

selective removal of excess stain from the tissue so that a specific substance may be stained distinctly from surrounding tissues

A

DIFFERENTIATION/DECOLORIZATION

29
Q

use of dyes which differentiate particular substances by staining them with a color that is different from that of the stain itself (metachromasia)

A

METACHROMATIC STAINING

30
Q

basic dyes belonging to the thiazine and triphenylmethane groups

A

METACHROMATIC STAINING

31
Q

stain tissues in color shades that are similar to the color of the dye itself

A

ORTHOCHROMATIC STAINING

32
Q

application of a different color or stain to provide contrast and background to the staining of structural components to be demonstrated

A

COUNTERSTAINING

33
Q

In the cytoplasmic stains, what is the color of eosin Y, Eosin B, Phloxine B?

A

Red

34
Q

In the cytoplasmic stains, what is the color of piciric acid, orange G, Rose Bengal?

A

Yellow

35
Q

In the cytoplasmic stains, what is the color of light green SF, Lissamine green?

A

Green

36
Q

Under nuclear stains, what is the color of neutral red, safranin 0, carmine, hematoxylin?

A

Red

37
Q

Under nuclear stains, what is the color of methylene blue, toluidine blue, Celestine blue?

A

Blue

38
Q

process where specific tissue elements are demonstrated, not by stains, but by colorless solutions of metallic salts which are thereby reduced by the tissue, producing an opaque, usually black deposit on the surface of the tissue

A

METALLIC IMPREGNATION

39
Q

agent is not absorbed by the tissue, but is held physically on the surface as a precipitate or as a reduction product

A

METALLIC IMPREGNATION

40
Q

selective staining of living cell constituents, demonstrating cytoplasmic structures by phagocytosis of the dye particle

A

VITAL STAINING

41
Q

done by injecting dye into any part of the body (intravenous, intraperitoneal or subcutaneous), producing specific color

A

INTRAVITAL STAINING

42
Q

stain living cells immediately after removal from the living body

A

SUPRAVITAL STAINING

HISTOPATH (26 decks)

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