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HISTOPATH > GLYCOGEN, CHON & NUCLEIC ACIDS > Flashcards

GLYCOGEN, CHON & NUCLEIC ACIDS Flashcards

1
Q

What are the stains under glycogen stains?

A

PAS
Best Carmine
Langhan’s Iodine

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2
Q

What is the color of PAS?

A

Magenta red

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3
Q

What is the color of PAS with Diastase control>

A

Red

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4
Q

What is the stain of choice for glycogen?

A

PAS with Diastase control

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5
Q

What is the color of Best Carmine?

A

Bright red

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6
Q

This stain is selective and specific for glycogen

A

Best Carmine

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7
Q

What are the stains under PROTEINS?

A

Alkaline-fast Green
Peracetic acid-Alcian Blue
Sakaguchi’s Test (Milton’s Reagent)

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8
Q

What is the color of Langhan’s iodine>

A

Mahogany brown

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9
Q

What is the color of Alkaline-fast Green?

A

Green

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10
Q

What is the color of Peacetic acid-Alcian Blue?

A

Blue-green

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11
Q

What is the color of Sakaguchi’s Test (Milton’s Reagent)?

A

Orange-red

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12
Q

This is used for histones and protamines.

A

Alkaline-fast Green

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13
Q

This is used for Cystine and Cysteine

A

Peracetic acid-Alcian blue

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14
Q

This is used for Arginine

A

Sakaguchi’s Test (Milton’s Reagent)

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15
Q

What are the stains under NUCLEIC ACIDS?

A

Feulgen Technique
Methyl Green-Pyronin
Fluorescein
Rhodamine
Acridine Orange
Acriflavine

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16
Q

Most reliable and specific stain for DNA

A

Feulgen Technique

17
Q

Most widely-used fluorochrome

A

Fluorescein

18
Q

What is the color of Feulgen Technique if used in DNA?

A

Red-purple

19
Q

What is the color of Methyl Green-Pyronin if used in DNA?

A

Green or Blue-green

20
Q

What is the color of Methyl Green-Pyronin if used in RNA?

A

Rose-Red

21
Q

What is the color of Fluorescein if used?

A

Apple green

22
Q

What is the color of Rhodamine if used?

A

Orange-red

23
Q

What is the color of Acridine orange if used in DNA and RNA?

A

Yellow-green
Brick to orange-red

24
Q

What is the color of acriflavine if used in DNA?

A

Yellow

HISTOPATH (26 decks)

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