LESSON 6: INFILTRATION AND EMBEDDING (CELLOIDIN PROCESSING) Flashcards
Purified pyroxylin nitrocellulose
CELLOIDIN PROCESSING
Suitable for specimens containing large cavities or hollow spaces which tend to collapse (eyes) & for larger embryos
CELLOIDIN PROCESSING
What is the disadvantage of Celloidin processing?
Tissues cannot be cut as thin as they are with paraffin wax
What is the advantage of celloidin processing?
Causes much less shrinkage & distortion; slow process
TRUE OR FALSE
Celloidin has a low viscosity with nitrocellulose?
TRUE
for bones, teeth large brain sections & whole organs
WET METHOD
Same with wet method EXCEPT for the following steps which are Gilson’s mixture and storage in alcohol is contradicted.
DRY METHOD
Provided superior results for light microscopic studies especially of hard tissues and samples for high resolution microscopy
PLASTICS
What are the types of plastic media?
EPOXY
POLYESTER
ACRYLIC
Slowest type of epoxy?
Araldite base (bisphenol)
Fastest type of epoxy?
Cyclohexene Dioxide (spurr)
For high resolution LM
ACRYLIC
valued for its hydrophilic nature
Glycol Methacrylate (GMA)
valued for its hardness
Methyl Methacrylate (MMA):
added as catalyst
Benzoyl Peroxide
