GENERAL STEPS IN FIXING SECTIONS ONTO SLIDE

Question 1

What are the general steps in fixing sections onto slide?

Answer 1

1. Adhesion
2. Floating on water bath
3. Fishing out
4. Orientation
5. Deparaffinization and Drying Sections
6. Post-mordanting

Question 2

performed to prevent washing out of tissue sections during staining

Answer 2

Adhesion

Question 3

is a substance which can be smeared onto the slides so that sections stick well to the slides

Answer 3

Adhesive

Question 4

Most commonly used

Answer 4

Mayer’s egg albumin

Question 5

Sections are dried and stored in 70% alcohol until it is ready for staining

Answer 5

Dried Albumin

Question 6

Added to the waterbath during flotation rather than applying it on slides

Answer 6

1% Gelatin

Question 7

slides are coated and allowed to dry at 37 OC for one hour or overnight prior to use

Answer 7

gelatin-formaldehyde mixture

Question 8

aqueous detergent diluted to 0.01% concentration

Answer 8

poly-L-lysine

Question 9

widely used in immunohistochemistry

Answer 9

poly-L-lysine

Question 10

Very useful in cytology, particularly for cytospin preparations of proteinaceous or bloody material

Answer 10

APES (3-aminoprophylthriethoxysilane)

Question 11

What is the temperature required when floating slides on water bath?

Answer 11

45-50 OC or 6-10 OC LOWER than the MP of wax

Question 12

to straighten tissue sections

Answer 12

Floating on water bath

Question 13

waterbath should have a temperature that is 10 OC lower than the melting point of the wax

Answer 13

Fishing-out

Question 14

correct positioning of the tissue section/ribbon on the slide

Answer 14

Orientation

Question 15

Secondary fixation (post-chroming)

Answer 15

Post-mordanting