STAINING LAB

Question 1

What are the types of hematoxylin dyes?

Answer 1

Aluminum
iron
Phosphotungstic acid
Copper

Question 2

TRUE OR FALSE:
Hematoxylin is not a natural dye

Answer 2

False: It is a natural dye

Question 3

This classification of dye is extracted from the core/heartwood of a Mexican tree (Hematoxylin Campechianum)

Answer 3

Hematoxylin

Question 4

Where is Hematoxylin extracted from?

Answer 4

Core/heartwood of a mexican tree known us Hematoxylin campechianum

Question 5

For a hematoxylin dye to ripen or oxidize, it should be?

Answer 5

Exposed to air and sunlight or to strong oxidizing agent.

Question 6

What are the types of Aluminum Hematoxylin?

Answer 6

Sodium iodate (Ehrlich’s)
Mercuric chloride (Harris’)
Alcoholic iodine (Cole’s)
Sodium iodate (Mayer’s)

Question 7

For this dye, the tissues are subjected to acid decalcification and acidic tissues

Answer 7

Sodium iodate (Ehrlich’s)

Question 8

For this dye it is used for routine nuclear staining, exfoliative cytology, and staining sex chromosomes.

Answer 8

Mercuric chloride (Harris’)

Question 9

For this dye, this is used for routine purposes especially in sequence to celestine blue

Answer 9

Alcoholic iodine (Cole’s)

Question 10

This dye is used for cytoplasmic glycogen

Answer 10

Sodium iodate (Mayer’s)

Question 11

What are the types of Iron hematoxylin?

Answer 11

Ferric ammonium chloride (Weigert’s)
Ferric ammonium sulfate (Heidenhain’s)

Question 12

This classification of iron hematoxylin is used for muscle fibers and connective tissues.

Answer 12

Ferric ammonium chloride (Weigert’s)

Question 13

This classification of iron hematoxylin is used for nuclear and cytoplasmic inclusions (chromatin. chromosomes, nucleoli, centromes, mitochondria) , voluntary muscle striations and myelin

Answer 13

Ferric ammonium sulfate (Heidenhain’s)

Question 14

What is the classification of phosphotungstic acid hematoxylin?

Answer 14

1% aq. phosphotungstic acid

Question 15

This dye is used for nuclei, fibrin, muscle striations, myofibrils, fibroglia, collagen, bone, cartilage, paraffin, celloidin and frozen section.

Answer 15

1% aq. phosphotungstic acid

Question 16

This dye is used for demonstration of spermatogenesis

Answer 16

Copper hematoxylin

Question 17

This dye is extracted from a female cochineal bug (coccus cacti)

Answer 17

Cochineal dyes

Question 18

where is cochineal dye extracted?

Answer 18

Female cochineal bug known as coccus cacti

Question 19

Cochineal dye is treated with _______ to produce ________.

Answer 19

Alum
Carmine

Question 20

This dye is a vegetable dye from lichens treated with ammonia and exposed to air

Answer 20

Orcein

Question 21

Orcein dye is from __________?

Answer 21

Lichens

Question 22

Orcein dye
For staining elastic fibers: ________.

Answer 22

Dark brown

Question 23

Synthetic dyes are ___________ or ___________.

Answer 23

Coal tar dyes or aniline dyes

Question 24

Synthetic dyes
Chromophores:___________
Auxochromes: ___________
Dye: __________________

Answer 24

Produces color
Promotes color retention
Chromophore + auxochrome

Question 25

Acid dyes: Picric acid
________________: Methylene blue
Neutral dyes: ____________,Giemsa,____________

Answer 25

Basic dyes
Romanowsky
Irishman

Question 26

What are the types of oil soluble dyes?

Answer 26

Sudan Black B
Sudan IV (Scharlach R)
Sudan III

Question 27

This oil soluble dye is the most sensitive and it stains phospholipids and neutral lipids

Answer 27

Sudan black B

Question 28

What is the color of Sudan Black B?

Answer 28

Black

Question 29

This oil soluble dye is recommended for staining neutral lipids

Answer 29

Sudan IV (Scharlach R)

Question 30

What is the color of Sudan IV ( Scharlach R)?

Answer 30

Red

Question 31

This oil soluble dye is the first to be introduced and it is used for fat stain for CNS tissues.

Answer 31

Sudan III

Question 32

What is the color of Sudan III when used?

Answer 32

Orange

Question 33

What are the other non-Lysochrome Lipid stain

Answer 33

Oil Red O
Osmic acid

Question 34

This dye is used for neutral fats and lipofuchsin (brilliant red)

Answer 34

Oil Red O

Question 35

This dye is used to stain lipids black

Answer 35

Osmic Acid