FROZEN SECTION Flashcards
This used for rapid diagnosis
Frozen section
Frozen section is used for the diagnostic and research of?
Enzyme histochemistry and demonstration of soluble substances
What type of staining is used in the Frozen section?
Immunofluorescent and immunohistochemical staining
In neuropathology, what stain is used?
Silver stain
This equipment in the Frozen section places tissue on a platform. Then, CO2 gas is used to freeze the tissue.
Microtome with CO2
How thick is the slice of a microtome with CO2?
5-10 um
This is a microtome that is mounted inside a cold chamber.
Cryostat
TRUE OR FALSE:
The Cryostat allows cutting thin tissue sections at low temperature.
TRUE
At what temperature is Cryostat being used?
-10 to -20’C
What are the advantages of Frozen sections?
A. Rapid processing time
B. Less equipment requirement
C. Less need for ventilation
What is the disadvantage of frozen section?
Relatively poor slide
What are the freezing reagents that are used in Frozen sections?
Liquid nitrogen
Isopentane called by liquid nitrogen
Carbon dioxide
Aerosol sprays
What is the disadvantage of Liquid nitrogen?
Soft tissues may crack and may snap-frozen
What is the other name for Aerosol sprays>
Cryowik
This term is used to describe the initial anoxic changes a tissue suffers when blood supply is cut-off.
Warm Ischemia
