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HISTOPATH > FIXATION 2.0 > Flashcards

FIXATION 2.0 Flashcards

1
Q

What fixative will be used for Lipids?

A

Mercuric chloride or Potassium Dichromate

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2
Q

What fixatives will be used for Phospholipids?

A

Baker’s formol calcium

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3
Q

What fixative will be used for Cholesterol?

A

Digitonin

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4
Q

What fixative will be used for CHO and CHON?

A

CHO: Alcohol fixatives
CHON: Neutral buffered formol-saline or formaldehyde vapors

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5
Q

Double fixation should be done at?

A

Electron microscopy

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6
Q

This is used for histochemical and enzyme study.

A

Aldehyde

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7
Q

chemical constituents of the cell

A

Metallic

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8
Q

Glycogen, pigments, blood and tissue films

A

Alcohol

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9
Q

This is used for acid mucopolysaccharides and connective tissue mucin

A

Lead

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10
Q

This fixes brain tissue in rabies

A

Acetone

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11
Q

This fixes fibrous tissues

A

Trichloroacetic acid

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12
Q

This fixes cytoplasmic structures

A

Osmium tetroxide

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13
Q

This fixes and precipitates nucleoproteins

A

Glacial acetic acid

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14
Q

This is used for the frozen tissue section and bacteriological smear.

A

Heat

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15
Q

What is the color of formaldehyde (formalin)

A

Colorless gas

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16
Q

This is a widely used fixative in histopathology today and is buffered at pH 7.

A

10% FORMALIN

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17
Q

This fixative preserves lipids but not CHO .

A

Formaldehyde (Formalin)

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18
Q

This is a fixative that is for simple microanatomical fixative.

A

10% Formol-saline

19
Q

This fixative is recommended for CNS, general post-mortem tissues, enzymes and nucleoproteins and demonstration of fats and mucin.

A

10% Formol-saline

20
Q

What is the fixation time of 10% formol-saline?

A

24 hours if 35°C
48 hours 20-25°C

21
Q

What is the other name of 10% Neutral Buffered Formalin?

A

Phosphate-Buffered Formalin

22
Q

This fixative is used for the preservation and storage of surgical, post mortem and research specimen.

A

10% Neutral Buffered Formalin/ Phosphate-Buffered Solution

23
Q

What is the formula of 10% Formal-Saline?

A

40% Formaldehyde + NaCl + Distilled water

24
Q

What is the formula for 10% Neutral buffered Solution?

A

Sodium dihydrogen phosphate + Disodium hydrogen phosphate + 40% Formaldehyde + Distilled water

25
Q

What is the fixation time of 10% Neutral Buffered Formalin?

A

4-24 hours

26
Q

This fixative is recommended for routine post-mortem tissues, Cytological structured and blood cell, Neutral fats and phospholipids.

A

Formol-corrosive

27
Q

What is the formula of Formal-Corrosive?

A

Saturated aq. Mercuric chloride + 40% Formaldehyde

28
Q

What is the fixation time of Formol-Corrosive?

A

3 - 24 hours

29
Q

This Fixative is used for rapid diagnosis and is recommended for Glycogen and micro-incineration technique.

A

Alcoholic Formalin (Gendre’s) Fixative

30
Q

What is formula of Alcoholic formalin?

A

95% ethyl alcohol saturated with picric acid + strong formaldehyde solution + Glacial acetic acid

31
Q

This fixative is recommended for electron microscopy and enzyme histochemistry

A

Glutaraldehyde

32
Q

What is the fixation time of Glutaraldehyde?

A

Small specimen: 30 - 2 hrs
Large specimen: 6-8 hrs up to 24 hours

33
Q

This is the most common metallic fixative and is widely used as a secondary fixative. May produce tissues that contain black ppt

A

Mercuric Chloride

34
Q

What is the formula for zenker’s fluid?

A

Mercuric Chloride + Glacial acetic acid

35
Q

This is used for small pieces of liver, spleen, connective tissue fibers and nuclei

A

Zenker’s fluid

36
Q

What is the fixation time of Zenker’s fluid?

A

12 - 24 hours

37
Q

What is the other name of Zenker-formol?

A

Helly’s solution

38
Q

This fixative is used for the pituitary gland, one marrow and blood containing organs including ctoplasmic granules.

A

Zenker-formol

39
Q

This fixative is used for the pituitary gland, one marrow and blood containing organs including ctoplasmic granules.

A

Zenker-formol

40
Q

What is the fixation time of zenker formol?

A

12 -24 hours

41
Q

It is an excellent cytologic fixative and is used for tumor biopsies of the skin.

A

Heidenhain’s Susa

42
Q

What is the fixation time of Heidenhain’s Susa?

A

3 - 12 hours

43
Q

This is used for bone marrow biopsies.

A

B - 5 Fixatives

44
Q

What is the fixation time of B -5 fixatives?

A

1 hr and 30 minutes - 2 hours

HISTOPATH (9 decks)

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