INFILTRATION Flashcards
What is infiltration?
- Removal of clearing agents from the tissue
- Replacing the clearing agent in a supportive medium
This is the process of completely filling the tissue spaces with a medium with a firm consistency.
Impregnation
What would be filled in the impregnation process?
Natural cavities
Spaces
Interstices
Under care and selection of waxes, what should be the volume of the medium?
25X tissue volume
Under care and selection of waxes, what should be the melting point?
56C
This type of tissue requires a higher melting point
Hard tissues
What is the type of microtome used under infiltration and impregnation?
Fixed-knife microtome
What type of knife is used under fixed-knife microtomes?
Heavier knives
This infiltrates support and encloses tissue specimens which provide structural integrity and shape of the tissue.
Embedding media
This encloses the tissue specimens for complete protection from the cutting of the tissues without damaging the tissue samples.
Embedding media
What should be the characteristic of an embedding media?
Must be capable of being converted readily from liquid to solid form.
What is commonly used in crystallization?
Paraffin and carbowax
Paraffin and carbowax are used in?
Crystallization
What is used in evaporation of the solvent?
Celloidin
Celloidin is used in?
Evaporation of the solvent
What is used in polymerization?
Plastics
Plastics are used in?
Polymerization
What are the mechanisms of solidification?
Crystallization
Evaporation
Polymerization
Enumerate the types of embedding
Paraffin embedding
This type of embedding is the most commonly used in routine tissue processing, and the best embedding medium.
Paraffin embedding
Paraffin embedding shrinks about _______ on cooling.
10%
Under paraffin embedding, what is the melting point for normal routine work?
56-58C
The paraffin oven that would be used under paraffin embedding should be set at what temperature?
2-5C higher than the melting point of paraffin wax.
What are the methods of paraffin impregnation?
Manual processing
Automatic processing
Vacuum processing