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HISTOPATH > LESSON 7: SECTIONING AND ADHESION OF TISSUE SECTIONS > Flashcards

LESSON 7: SECTIONING AND ADHESION OF TISSUE SECTIONS Flashcards

1
Q

a previously processed tissue, is trimmed and cut into uniformly thin slices or sections

A

SECTIONING

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2
Q

this procedure is also referred to as microtomy

A

SECTIONING

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3
Q

What are the 3 basic parts of microtome?

A
  1. BLOCK HOLDER
  2. KNIFE CARRIER AND KNIFE
  3. PAWL, RATCHET FEED WHEEL AND ADJUSTMENT SCREWS
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4
Q

where the embedded tissue is held in position

A

BLOCK HOLDER

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5
Q

perform the actual cutting of tissue sections

A

KNIFE CARRIER AND KNIFE

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6
Q

line up tissue block in proper position with the knife, adjust proper thickness

A

PAWL, RATCHET FEED WHEEL AND ADJUSTMENT SCREWS

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7
Q

What are the types of Microtome?

A

ROCKING
ROTARY
SLIDING
ULTRATHIN
FREEZING

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8
Q

*simplest microtome
*for small and large paraffin-embedded blocks
*not for serial sections

A

ROCKING

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9
Q

*most common microtome
*for paraffin embedded blocks

A

ROTARY

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10
Q

*most dangerous

A

SLIDING

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11
Q

What are the types of Sliding microtome?

A

STANDARD SLIDING
BASE-SLEDGE

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12
Q

STANDARD SLIDING: celloidin
BASE-SLEDGE: ________________

A

hard and tough tissue blocks in all forms of media

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13
Q

*for electron microscopy
*small specimen fixed in osmium tetroxide and embedded in plastic

A

ULTRATHIN

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14
Q

*undehydrated tissues in frozen state
*fats and heat sensitive tissue constituents

A

FREEZING

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15
Q

rotary microtome inside a cold chamber

A

COLD MICROTOME/CRYOSTAT

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16
Q

Cold microtome/Cryostat is maintained between __________?

A

-5 to -30 OC (ave. is -20 OC)

17
Q

between the edge of the knife & the tissue block

A

Clearance angle

18
Q

angle of cutting

A

Wedge angle

19
Q

angle of cutting facet

A

Bevel angle

20
Q

What are the angles of clearance, wedge and bevel?

A

Clearance angle (5-10 degrees)
Wedge angle (15 degrees)
Bevel angle (27-32 degrees)

21
Q

The purpose of this is the Removal of gross nicks and blemishes

22
Q

Removal of burrs, for final polishing and sharpening of the cutting edge

23
Q

Heel-to-Toe, edge first

24
Q

Toe-to-Heel, edge last

25
Q

20-30 strokes in each direction
10-20 strokes each surface (Minot and Plane-wedge knives)

26
Q

40-120 double strokes

27
Q

What are the other honing procedures?

A

Plate Glass Honing
Factory Grinding
Automatic hones

28
Q

used for final polishing

A

Diamantine:

HISTOPATH (26 decks)

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