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Clinical Chemistry > Specimen Preparation, Handling, Transport & Storage > Flashcards

Specimen Preparation, Handling, Transport & Storage Flashcards

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1
Q

Preanalytical Variable in Px. Preparation

A

Age & Gender
Diurnal Variation
Posture
Fasting
Diet
Drugs
Stress

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2
Q

Exercise - Transient Increase

A

Lactate, FA, Ammonia

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3
Q

Fasting Specimens

A

FBS
GTT
Lipid Profile
Gastrin
Insulin
Aldoterone/Renin

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4
Q

Over fasting - 72 hrs

A

Increased in Males: TAG
Decreased in Females: 45mg/dl of Glucose

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5
Q

Increased during standing

A

Albumin
Cholesterol
Aldosterone
Calcium

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6
Q

Increased - From Supine to Sitting

A

Albumin
Enzymes
Calcium

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7
Q

Increased - From Sitting to Supine

A

Proteins
Lipids
BUN
Iron
Calcium

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8
Q

Increased - Prolonged tourniquet application

A

Potassium
Proteins
Enzymes
Lactate
Cholesterol
Ammonia

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9
Q

Increased - Fist Clenching

A

Potassium
Phosphate
Lactate

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10
Q

Analytes Increased in AM

A

ACTH
Iron
Cortisol

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11
Q

Analytes Increased in PM

A

GH
ACP

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12
Q

Calcium is ___& decrease supine

A

4%

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13
Q

Iron decreases up to ___% during the day.

A

30%

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14
Q

Decreased cortisol level by 8:00 pm

A

50%

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15
Q

Uric acid levels peak in men in their

A

20s

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16
Q

Increase of Cholesterol/TAG per year

A

TG - 2mg/dL (0.02 mmol/L)
TC - 2 md/dL (0.05 mg/dL)

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17
Q

Sleeping patients

A

Should be awakened

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18
Q

Outpatient/Ambulatory Patient is counterchecked with:

A

Driver’s License or Valid ID with photo

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19
Q

3 ways of ID

A

Positive ID: Verbal ID statement
Passive ID: Check ID band
Comparison of labeled specimen with px. ID before leaving

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20
Q

Average human blood

A

5 quartz (4.73 L)

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21
Q

Venous Blood

A

Deoxygenated, Dark Red Color

22
Q

Most Common Site for Venipuncture

A

Antecubital fossa - (1) Median (2) Cephalic (3) Basilic

23
Q

CLSI Recommended site for Venipuncture

A

Median Cubital Vein

24
Q

international Standard - A backflow is first seen in the ______________

A

Flash window of the Needle hub

Note: If no flash window in the choices, answer - needle hub

25
Q

Most common Antiseptic

A

70% Alcohol

26
Q

Used for Ethanol Testing

A

Benzalkonium chloride/Zephiran Chloride (1:750)

27
Q

CLSI recommended - blood culture, Infants, Iodine Sensitivity

A

Chlorhexidine gluconate

28
Q

Tourniquet Application

A

3-4 (7.5-10 cm) inches ABOVE the site for <1 minute

29
Q

CLSI - Tourniquet Reapplication

A

2 minutes

30
Q

Bacteria transmitted due to Reusable tourniquets

A

MRSA - Methicillin-resistant Staphylococcus aureus

31
Q

10% contamination of 5% glucose increase glucose by _____ mg/dL

A

500 mg/dL

32
Q

Needle Angle (Veni)

A

<30 degrees
15-30 degree

33
Q

Needle - Yellow Cap gauge

A

20 gauge

34
Q

Needle - Green Cap Gauge

A

21 gauge

35
Q

Needle - Black Cap Gauge

A

22 gauge

36
Q

Standard gauge for Venipuncture

A

21 gauge

37
Q

Pediatric/Small veins needle gauge

A

23 gauge

38
Q

Blood Letting Gauge

A

16-G

39
Q

Lumbar puncture needle gauge

A

18-G

40
Q

25-G

A

Infants/Neonates/Blood from Scalp needle Gauge

41
Q

Routine Needle Length

A

1-1.5 inches

42
Q

Butterfly Needle Length

A

1/2 - 3/4 (0.5-0.75) inch

43
Q

Cause of Hemolysis

A

Prolong tourniquet application
Alcohol contamination
Small needle bore
Agitation
Frothing/Bubbling

44
Q

Lancet Length

Disposable: ________
Non-disposable: ______
Rodriguez - _____
NCBI - _____

A

Lancet Length

Disposable: 0.75 mm
Non-disposable:
1.75 mm (Rodriguez)
2.8 - 3.2 mm (NCBI)

45
Q

Most Routine Site for Capillary Puncture

A

4th Finger

46
Q

Sites to avoid in Capillary Puncture

A

Thumb
5th Finger

47
Q

Depth of Puncture

Children/Adults

A

<2.0/2-2.5 mm

48
Q

Detected analytes in Arterialized Capillary Blood

A

pH
pCO2

49
Q

Preferred site for Arterialized Capillary Blood

A

Earlobe

50
Q

First microcollection tube to be filled in Skin puncture

A

EDTA/Lavander

51
Q

Order of Draw - Skin Puncture

A

(B)lood gases
(S)lides
(ED)TA
(O)ther microcollection tubes
(S)erum container

Clinical Chemistry (20 decks)

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