Instrumentation Flashcards

1
Q

Most common Glassware

A

Borosilicate Glass

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2
Q

Temperature Rate of Borosilicate Glass

A

515 C

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3
Q

Borosilicate Glass has high ____________ resistance, but low __________ resistance.

A

High Thermal Resistance
Low Alkali Resistance

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4
Q

Boron-free Glass

A

a.k.a Soft Glass
High Alkali Resistance

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5
Q

What contains Alkali Resistant Glass?

A

Magnesium Lime

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6
Q

Pyrex, Kimax

A

Borosilicate Glass

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7
Q

Specialized alumina Silicate Glass

A

Coring

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8
Q

Corex is ______ times stronger than borosilicate glass

A

Six (6)

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9
Q

Temperature rate of Vycor

A

900 C

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10
Q

Vycor

A

High thermal drastic-heat shock resistance

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11
Q

Vycor is both _______ and _______ resistant

A

Acid and Alkali

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12
Q

Corex is strengthen ___________ than __________.

A

Strengthen CHEMICALLY than Thermally

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13
Q

It is used for Disposable glasswares

A

Flint Glass/Soda Lime Glass

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14
Q

Most Common and Simplest type of Pipette

A

Glass Pipet

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15
Q

Enumerate Classification of Pipette

A

According to Calibration
1. To Contain
2, To Deliver

According to Drainage Characteristic
1. Blow Out
2. Self-draining

According to Purpose
1. Transfer Pipets
- Volumetric
- Ostwald-folin
- Pasteur
- Automatic micropippete
2. Measuring/Graduated Pipets
- Serologic
- Mohr
- bacteriologic
- Ball, Kolmer, Kahn Pipets
- Micropipettes

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16
Q

Transfer pipet that is used for NON-VISCOUS fluid and is SELF DRAINING

A

Volumetric Pipet

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17
Q

Ostwald Folin

A

Viscous fluid and Blow out

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18
Q

Pasteur pipet

A

Transfer fluid without consideration of specific volume

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19
Q

Automatic Micropipette

A

Most sensitive

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20
Q

Serologic pipet has ____________ to the tip and is ___________.

A

Graduation to the tip
Blow Out

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21
Q

Mohr pipet has ___________ to the tip and is ____________.

A

No graduation to the tip
Self-draining

22
Q

Recall: An automated pipet that rely on PISTON FOR SUCTION and DISPOSABLE TIPS.

A

Air Displacement Pipette

23
Q

Recall: An automated pipet that rely by MOVING THE PISTON in the pipet tip and has PERMANENT TIP.

A

Positive Displacement Pipet

24
Q

Micropipette Tips are made from ____________.

A

Polypropylene

25
Q

Cleaning solution for Glasswares

A

Acid Dichromate

26
Q

Recall: Polypropylene

A

Autoclaved
Common Micropipette tips/Plastic Pipette tips

27
Q

Used for Plastic test tube and Test Tube Rack

A

Polycarbonate

28
Q

4 Basic Approaches to Automation

A

Continuous Flow
Centrifugal Analyzer
Discrete Analyzer
Dry Slide

29
Q

Continuous Flow Analyzer

A

Reagents, diluents and samples are pumped through a system of CONTINUOUS TUBING

Mixing: Glass Coil

30
Q

Uses Batch Testing Analysis

A

Centrifugal analyzer

31
Q

Centrifugal Analyzer

A

One test per sample but loaded at the same time.

Mixing: Spinning Rotor (1000 rpm)

32
Q

Used for STAT

A

Discrete Analyzer

33
Q

Discrete Analyzer

A

Measures only the test requested on a sample and each sample is analyzed is separately in their own reaction container.

Mixing: Magnetic stirring bar

34
Q

Batch Testsing

A

All sample are loaded at the same but one single test is conducted per sample.

35
Q

Parallel Testing

A

Simultaneous analysis

36
Q

Random Access Tesing

A

Any sample, any test, any sequence

37
Q

Sequential Testing

A

Multiple test are analyzed one after another each specimen

38
Q

Open Reagent System

A

Can use other than manufacturer’s reagent

39
Q

Close Reagent System

A

Only the manufacturer’s reagent can be used

40
Q

Time of Pipette Calibration in a year

A

3-4 times/year

41
Q

Class ___ pipet do not require Calibrations.

A

Class A

42
Q

QC: Used to verify pipette volume and accuracy

A

Gravimetric and Spectrometric Method

43
Q

Calibrating medium - TD Pipette

A

Distilled H2O

44
Q

Calibrating medium - TC Pipette

A

Mercury

45
Q

Used to compare reproducibility of Pipet tips brands

A

0.1% Phenol Red solution in distilled H2O

46
Q

Serves as separation and cleaning in Continuous Flow analyzers

A

air bubbles

47
Q

Rapid and Ideal Centrifuge

A

Fixed Angle Head

48
Q

Horizontal Head

Spinning:
Not spinning:

A

Horizontal Head

Spinning: Horizontal
Not spinning: Vertical

49
Q

Type of Reagent Water

Type I:
Type II:
Type III:

A

Type of Reagent Water

Type I: Standard solution
Type II: Reagent Preparation
Type III: Washing

50
Q

Types of water used for consumption and cooking ?

a. distilled
b. potable
c. purified
d. AOTA

A

B. Potable

51
Q

Most popular and versatile analyzer

A

Discrete Analyzer

52
Q

Minimum volume of Discrete Analyzer

A

2-6 ul