Instrumentation Flashcards
Most common Glassware
Borosilicate Glass
Temperature Rate of Borosilicate Glass
515 C
Borosilicate Glass has high ____________ resistance, but low __________ resistance.
High Thermal Resistance
Low Alkali Resistance
Boron-free Glass
a.k.a Soft Glass
High Alkali Resistance
What contains Alkali Resistant Glass?
Magnesium Lime
Pyrex, Kimax
Borosilicate Glass
Specialized alumina Silicate Glass
Coring
Corex is ______ times stronger than borosilicate glass
Six (6)
Temperature rate of Vycor
900 C
Vycor
High thermal drastic-heat shock resistance
Vycor is both _______ and _______ resistant
Acid and Alkali
Corex is strengthen ___________ than __________.
Strengthen CHEMICALLY than Thermally
It is used for Disposable glasswares
Flint Glass/Soda Lime Glass
Most Common and Simplest type of Pipette
Glass Pipet
Enumerate Classification of Pipette
According to Calibration
1. To Contain
2, To Deliver
According to Drainage Characteristic
1. Blow Out
2. Self-draining
According to Purpose
1. Transfer Pipets
- Volumetric
- Ostwald-folin
- Pasteur
- Automatic micropippete
2. Measuring/Graduated Pipets
- Serologic
- Mohr
- bacteriologic
- Ball, Kolmer, Kahn Pipets
- Micropipettes
Transfer pipet that is used for NON-VISCOUS fluid and is SELF DRAINING
Volumetric Pipet
Ostwald Folin
Viscous fluid and Blow out
Pasteur pipet
Transfer fluid without consideration of specific volume
Automatic Micropipette
Most sensitive
Serologic pipet has ____________ to the tip and is ___________.
Graduation to the tip
Blow Out
Mohr pipet has ___________ to the tip and is ____________.
No graduation to the tip
Self-draining
Recall: An automated pipet that rely on PISTON FOR SUCTION and DISPOSABLE TIPS.
Air Displacement Pipette
Recall: An automated pipet that rely by MOVING THE PISTON in the pipet tip and has PERMANENT TIP.
Positive Displacement Pipet
Micropipette Tips are made from ____________.
Polypropylene
Cleaning solution for Glasswares
Acid Dichromate
Recall: Polypropylene
Autoclaved
Common Micropipette tips/Plastic Pipette tips
Used for Plastic test tube and Test Tube Rack
Polycarbonate
4 Basic Approaches to Automation
Continuous Flow
Centrifugal Analyzer
Discrete Analyzer
Dry Slide
Continuous Flow Analyzer
Reagents, diluents and samples are pumped through a system of CONTINUOUS TUBING
Mixing: Glass Coil
Uses Batch Testing Analysis
Centrifugal analyzer
Centrifugal Analyzer
One test per sample but loaded at the same time.
Mixing: Spinning Rotor (1000 rpm)
Used for STAT
Discrete Analyzer
Discrete Analyzer
Measures only the test requested on a sample and each sample is analyzed is separately in their own reaction container.
Mixing: Magnetic stirring bar
Batch Testsing
All sample are loaded at the same but one single test is conducted per sample.
Parallel Testing
Simultaneous analysis
Random Access Tesing
Any sample, any test, any sequence
Sequential Testing
Multiple test are analyzed one after another each specimen
Open Reagent System
Can use other than manufacturer’s reagent
Close Reagent System
Only the manufacturer’s reagent can be used
Time of Pipette Calibration in a year
3-4 times/year
Class ___ pipet do not require Calibrations.
Class A
QC: Used to verify pipette volume and accuracy
Gravimetric and Spectrometric Method
Calibrating medium - TD Pipette
Distilled H2O
Calibrating medium - TC Pipette
Mercury
Used to compare reproducibility of Pipet tips brands
0.1% Phenol Red solution in distilled H2O
Serves as separation and cleaning in Continuous Flow analyzers
air bubbles
Rapid and Ideal Centrifuge
Fixed Angle Head
Horizontal Head
Spinning:
Not spinning:
Horizontal Head
Spinning: Horizontal
Not spinning: Vertical
Type of Reagent Water
Type I:
Type II:
Type III:
Type of Reagent Water
Type I: Standard solution
Type II: Reagent Preparation
Type III: Washing
Types of water used for consumption and cooking ?
a. distilled
b. potable
c. purified
d. AOTA
B. Potable
Most popular and versatile analyzer
Discrete Analyzer
Minimum volume of Discrete Analyzer
2-6 ul
