Special culture requirements (Pg. 123) - C

Question 1

Media used for isolation / Media content

H influenzae (Media used for isolation / Media content)

Answer 1

Chocolate agar / Factors V (NAD+) and X (hematin)

Question 2

Media used for isolation / Media content

N gonorrhoeae, N meningitidis (Media used for isolation / Media content)

Answer 2

• Thayer-Martin agar

“Very Typically Cultures Neisseria”

• Selectively favors growth of Neisseria by inhibiting growth of gram ⊕ organisms with VANCOMYCIN, gram ⊝ organisms except Neisseria with TRIMETHOPRIM and COLISTIN, and fungi with NYSTATIN

Question 3

Media used for isolation / Media content

B pertussis (Media used for isolation / Media content)

Answer 3

• Bordet-Gengou agar (Bordet for Bordetella)
  Regan-Lowe medium
• Potato extract
  Charcoal, blood, and antibiotic

Question 4

Media used for isolation / Media content

C diphtheriae (Media used for isolation / Media content)

Answer 4

• Tellurite agar, Löf er medium

Question 5

Media used for isolation / Media content

M tuberculosis (Media used for isolation / Media content)

Answer 5

• Löwenstein-Jensen agar

Question 6

Media used for isolation / Media content

M pneumoniae (Media used for isolation / Media content)

Answer 6

• Eaton agar

- Requires cholesterol

Question 7

Media used for isolation / Media content

Lactose-fermenting enterics (Media used for isolation / Media content)

Answer 7

• MacConkey agar

- Fermentation produces acid, causing colonies to turn pink

Question 8

Media used for isolation / Media content

E coli (Media used for isolation / Media content)

Answer 8

• Eosin–methylene blue (EMB) agar

- Colonies with green metallic sheen

Question 9

Media used for isolation / Media content

Legionella (Media used for isolation / Media content)

Answer 9

• Charcoal yeast extract agar buffered with cysteine and iron

Question 10

Media used for isolation / Media content

Fungi (Media used for isolation / Media content)

Answer 10

• Sabouraud agar

“Sab’s a fun guy!”