signal trans 3 part 3

Question 2

clathrin polymerization accompanied by

Answer 2

vacuole formation in Enzyme-Linked Receptor term.

Question 3

Release of — —-vesicle into cytoplasm

 Shedding of ——- ———

 Fusion of vesicle with ——– (internal —– ph)

Answer 3

Release of clathrin-coated vesicle into cytoplasm

Shedding of clathrin coat  Fusion of vesicle with endosome (internal acidic ph

3rd step in Enzyme-Linked Receptor

Question 5

tf in Enzyme-Linked Receptor: Termination

Potential recycling of receptors to plasma membrane is not possible

Answer 5

F

recycling happens at end

and

Transfer of remaining contents to lysosomes for degradation

Question 6

tf Cytokine cant recruit manyintracellular signaling proteins

Answer 6

F it can recruit a broad range because of itts great diversity

Question 7

tf the intracellular enzymatic activity has no intrincic enzymatic activity

Answer 7

t

Question 8

single polypeptide

Answer 8

cytokine receptor

Question 9

1st step of Cytokine Receptor action

Answer 9

Cytokine binding Dimerization

JAK activation and cross-phosphorylation Subunit phosphorylation

Question 10

Cytokine receptor

Answer 10

multimeric complexes

Question 12

STAT binding

Answer 12

prompts Phos Jak to phosphorylate it

Question 13

2 thing phos in cytokine receptor activation

Answer 13

JAKS and subunits

Question 14

upon sTAT phosphorylation

Answer 14

STAT dimerize

AND

Translocation to nucleus Altered gene expression

Question 15

Protein phosphatases remove —- phosphates from the receptor and/or activated —–

Answer 15

Protein phosphatases remove tyrosine phosphates from the receptor and/or activated STATs.

Cytokine receptor termination

Question 16

SOCS (suppressor of cytokine signaling)

Answer 16

inhibit STAT phosphorylation by binding

inhibiting JAKs or competing with STATs for phosphotyrosine binding sites

Question 17

Multimeric formation of the receptor after ligand binding triggers —– of the ligand-receptor complex.

Answer 17

Multimeric formation of the receptor after ligand binding triggers endocytosis of the ligand-receptor complex.

cytokine receptor termination

Question 18

importin α5

Answer 18

subunit of importin α5-β complex

Question 19

Ran-GTP

Answer 19

dissociates

STAT1 dimer (NLS) and importin α5 complex

(happens in nucleus)

Question 20

after dissociation of STAT 1 and importin a5

the STAT1 dimer

Answer 20

binds to DNA targets

and causes Expression of antiviral response

Question 21

VP24 protein from EBola

Answer 21

Competes with STAT1 dimer for binding site on importin α5 subunit

Question 22

VB24 and importin α5 subunit complex

Answer 22

diffuse thru nuclear pore

and Dissociated by complex by Ran-GTP

Question 23

Suppression of antiviral response

Answer 23

promoted by VP24 protein after Dissociation from complex by Ran-GTP

Question 24

Small KD

Answer 24

Receptor has high affinity for ligand.

Question 25

Kd=

Answer 25

koff/ kon

Question 26

Free = KD,

Answer 26

then Bound = 0.5 Bmax

Question 27

LR=

Answer 27

([R]o [L]o) /(KD + [L]o)

Question 28

Bound

Answer 28

B max(Free/Free+Kd)

Question 30

if free>Kd

Answer 30

bound= bmax

Question 31

Negligible ligand depletion (bound < 10% of free)  Negligible inactivation of ligand and receptor

Answer 31

2 conditions of saturation binding relation

Question 32

Label the saturation plot

Answer 32

Question 33

If EC50 >KD

Cell expresses more receptors than required for effective biological responses.

Answer 33

F

expresses less

EC50 is Half-Maximal Effective Conc

Question 34

tf 2 conditions of saturation bidning relation all ligand and receptor act and there are few cell surface interaction

Answer 34

F

Negligible inactivation of ligand and receptor

Negligible cell surface interactions

Question 35

main condition of saturation plot

Answer 35

Equilibrium conditions

Question 36

what does this indicate abt parotid

Answer 36

KD are same but control has more Bmax than parotid

meaning;

binding characteristics of salivary gland muscarinic acetylcholine receptors of the parotid contribute to reduced saliva stimulation

Question 37

what does this indicate abt SM gland

Answer 37

Diabetic has greater KD

but = B max

no conconclusion on whether

binding characteristics of salivary gland muscarinic acetylcholine receptors of SM gland contribute to reduced saliva stimulation or not?

Question 38

Homogeneous,——-(1:1) populations of ligand and receptor

Answer 38

Homogeneous, monovalent (1:1) populations of ligand and receptor

Saturation Plot

Question 41

advantage of scatchard plot

— evaluation is easy for checking original — (straight line) or comparing different — or receptors.

Answer 41

Visual evaluation is easy for checking original assumptions (straight line) or comparing different ligands or receptors.

Question 42

—- on both axes magnifies experimental error.

Answer 42

“Bound” on both axes magnifies experimental error.

disadvantage of scatchard plot