CT 3 part 1

Question 2

signals from the outside of the cell to stimulate gene transcription of Collagen

Answer 2

tgf beta il 1b

Question 3

tnf alpha

Answer 3

signals from the outside of the cell to inhibit gene transcription of Collagen

Question 4

TGF beta binds to

Answer 4

ser thre kinase activity

Question 5

after ser thre kinase activity is triggered by TGF beta

Answer 5

SMAD 2 and 3 phosphorylate

Question 6

phosphorylated SMAD 2 and 3

Answer 6

agg with SMAD 4 to form heterometric complex

Question 7

heterometric complex

Answer 7

go to nucleus and act transcription of collagen

Question 8

SMAD 7,8 inh

Answer 8

phos of SMAD 2 and 3

Question 9

heterometric complex made from SMAD 2 and 3

Answer 9

binds CIS-elements in the 5′-flanking region for the regulation of transcription of the COL1A2 gene

Question 10

collagen gene

Answer 10

repressors and enhancers

Question 11

COL1 a1 controlled by

Answer 11

TGF beta act. protein

Question 12

ColagenMolecule is to be secreted; it’s synthesized on —- —– ribosomes as larger precursor

Answer 12

Collagen Molecule is to be secreted; it’s synthesized on membrane-bound ribosomes as larger precursor

Question 13

pre pro alpha chain (collagen)

Answer 13

hydrophobic and can be thread thru lumen of ER

Question 14

pre pro alpha chain (collagen)

Answer 14

cotransltionally cleaved

Question 15

signal peptidase

Answer 15

cleave N and C terminal

Question 17

cleaveage of N and C terminal

Answer 17

leaves a telopeptide

Question 18

contains intrachain disulfides

Answer 18

pN; N-terminal propeptide

Question 19

contains interchain disulfides

Answer 19

pC; C-terminal propeptide

Question 20

Collagens are modified

Answer 20

Collagens are modified after the amino acids are incorporated into the growing polypeptide chain

Question 21

prolyl residues and lysyl residues

Answer 21

are hydroxylated

Question 22

Glycosylation of—– residues

Answer 22

Glycosylation of hydroxylysyl residues

Question 23

Prolyl hydroxylase has — ion at its active site

Answer 23

Prolyl hydroxylase has Fe++ ion at its active site

Question 24

Prolyl hydroxylase

Answer 24

Prolyl hydroxylase is a dioxygenase

Question 25

requires ascorbate

Answer 25

Prolyl hydroxylase

Question 26

prolyl residue is added with —– —

and —to be hydrozylated

Answer 26

alpha ketogluterate and O2

Question 27

Prolyl hydroxylase

Answer 27

act O2

Question 28

Proline is hydroxylated at —-

by the action of prolyl hydroxylase

Answer 28

Proline is hydroxylated at C-4

by the action of prolyl hydroxylase

Question 29

TF Free proline is a substrate

Answer 29

Free proline is NOT a substrate

Question 30

Hydroxylation at C-4 only if Pro is on the —– side of a Gly residue in the (Gly-XY)n sequence in collagen

Answer 30

Hydroxylation at C-4 only if Pro is on the amino side of a Gly residue in the (Gly-XY)n sequence in collagen

Question 31

Different enzyme; hydroxylation at —- if Pro is on the carboxyl side of a Gly in the (Gly-X-Y)n sequence in collagen

Answer 31

Different enzyme; hydroxylation at C-3 if Pro is on the carboxyl side of a Gly in the (Gly-X-Y)n sequence in collagen

3-prolyl hydroxylase

Question 32

Dietary deficiency of vitamin C

Answer 32

Scurvy

Question 33

Poor wound healing

Poor calcification of developing teeth

Answer 33

Vit C

Question 34

Fragile blood vessels

Suppressed bone growth

Answer 34

Scurvy

Question 35

gingival bleeding and

Skin lesions

Answer 35

deficiency of vitamin C

Question 36

Hydroxylation of lysine only if it’s on the amino side of a glycine residue

Answer 36

Specificity of lysyl hydroxylase

Question 37

O2 and ascorbate

Answer 37

needed for lysyl hydroxylase to work

Question 38

α ketoglutarate; succinate

Answer 38

reactant and substrtae for lysyl hydroxylase and prolyl hydroxylase

Question 39

Ehlers-Danlos Syndrome,

Answer 39

Deficiency of lysyl hydroxylase

Question 40

❂ Hyperextensibility of skin and joints

Answer 40

Ehlers-Danlos Syndrome,