CT 3 part 2 Flashcards
tf Ehlers-Danlos Syndrome, has inc crossliking of collagen
F
lowered
Poor wound healing
and Musculoskeletal deformities
Ehler danslos
Hyp
CANNOT be incorporated into a growing polypeptide chain so addition of [14C] Hyp will not result in formation of [14C] collagen
Proline and hydroxyproline are both radioactive but
Hyp CANNOT be incorporated into a growing polypeptide chain
Unique to collagens;
Unique to collagens; glycosylated hydroxylysyl residues
2 types of O linked glycosylations
serine and 5 hydroxylysine
asparagine
N glycosyl Linkage
glycosylations of
glucose and galactose
After post-translational modification, the 3 polypeptides associate to form the procollagen molecule.
After post-translational modification, the 3 polypeptides associate to form the procollagen (Type I procollagen) molecule.
where is procollagen made
in the ER lumen
Extension peptides
help guide the formation of the triple helix.
Each polypeptide is —- through a large portion of the molecule and stabilized partly by —— of the pyrrolidone rings of proline.
Each polypeptide is helical through a large portion of the molecule and stabilized partly by steric repulsion of the pyrrolidone rings of proline.
3 polypeptides (Pro α Chains) wind around each other to form a —- —–, with —— bonds between individual chains
3 polypeptides (Pro α Chains) wind around each other to form a —- —–, with —— bonds between individual chains
procollagen
Residues in each chain are —— staggered such that a gly, an X and a Y from ——chains are on the same level along the helix axis.
Residues in each chain are vertically staggered such that a gly, an X and a Y from different chains are on the same level along the helix axis.
procollagen
stiff cable
Tm is the temperature at
temperature at which 1/2 of the helical structure is lost
abnormal collagen
smaller Tm
