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B&M Exam 2 > Salmonella > Flashcards

Salmonella Flashcards

1
Q

gram
shape
lactose fermenter?
urease
TSI – smell, color
motile?
hemolysis?

A

gram negative
rod
lactose non fermenter
urease negative
H2S gas on TSI – rotten egg smell, black colonies
motile by peritrichous flagella
gamma hemolysis on blood agar

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2
Q

culture media

A

macconkey
eosin methylene blue
brilliant green
salmonella and shigella agar

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3
Q

which culture media are selective for salmonella

A

brilliant green
salmonella and shigella agar

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4
Q

habitat

A

normal digestive tract flora

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5
Q

transmission route
trasnmission of salmonella pullorum

A

oral fecal
transovarial in poultry – salmonella pullorum

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6
Q

which salmonella are non motile

A

s gallinarum and s pullorum

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7
Q

salmonella enterica subspecies

A

enterica I
salamae II
arizonae IIIa
diarazonae IIIa
houtane IV
indica VI

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8
Q

typhoidal enterica I

A

s typhi
s paratyphi

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9
Q

non typhoidal enterica I

A

s typhanimurium
s enteritidis

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10
Q

in cold blooded animals and the environemtn

A

salmonella enterica subsepcies
salamae II
arizonae IIIa
diarazonae IIIa
houtane IV
indica VI
salmonella bongori subspecies
subspecies V

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11
Q

host specific enterica I serotypes

A

typhi, paratyphi, sendal – humans
typhisuis – pigs
abortusovis – sheep
abortusequi – horse
gallinarum, pullorum – poultry
typhimurium DT2 – pigeons

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12
Q

host adapted subspecies of enterica I serotypes

A

cholerasuis – pigs
dublin – cows

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13
Q

zoonotic potential?

A

all salmonella excepts s gallinarum and s pullorum (specialized for birds)

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14
Q

which 3 salmonella are part of the 7 poultry priority diseases

A

s pullorum
s gallinarum
s enterica var enteritidis

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15
Q

what is different about s gallinarum and s pullorum

A

non motile, no flagella
specialized for birds, non zoonotic

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16
Q

what species is salmonella devastating in

A

poultry
equine

17
Q

adhesion structures

A

fimbriae (pili)
5-10 flagella
capsule
LPS (endotoxin) – lipid A – causes gangrene

18
Q

enzymes

A

catalase
superoxide dismutase – protect against ROS
siderophores – rob iron

19
Q

toxins

A

genome or plasmids at pathogenicity islands
T3SS
adhesins
invasins
hemagglutinins
exotoxins

20
Q

what does the intracellular invasion

A

T3SS-1

21
Q

what induces intracellular multiplication in salomella containign vacuoles in the cytosol

A

T3SS-2

22
Q

what is T3SS-1 encoded on, what does it do

A

encoded on SPI-1
inject virulence proteins into host cells, transfer bacterial factors for host cell invasion

23
Q

what is T3SS-2 encoded on, what does it do

A

encoded on SPI-2
transfer effector proteins for intracellular survival and multiplication

24
Q

what helps with intracellular invasion and spread to other cells

A

SCV formation in cytosol
T3SS-2
host actin filament deposition around salmonella using SifA

25
Q

how does reinfection occur

A

bile excretion

26
Q

majority of animals have what kind of infection

A

silent

27
Q

disease in young poultry and by which species

A

viscous white diarrhea pasted at anal region
s pullorum

28
Q

disease in old poultry and by which species

A

watery to mucoid yellow diarrhea
anemia
pale shrunken combs and wattles
S gallinarum (fowl typhoid)

29
Q

diagnosis of salmonella

A

fecal cultuer followed by biochemical tests

30
Q

treatment of salmonella

A

replace los fluids, electrolytes, acid base balance

31
Q

why is antimicrobial use controversial

A

cure rate low
risk of inducing carrier animals

32
Q

why would you use antimicrobials

A

systemic infection

33
Q

control and prevention

A

control of fecal contamination

34
Q

vaccines

A

attenuated live against s gallinarum and s pullorum
killed against s enteritidis in europe

35
Q

medically important s enterica subspecies

A

s enterica subspecies enteric I

36
Q

what organ do they prefer

A

intestines
but can breach intestinal wall to infect other organs

B&M Exam 2 (31 decks)

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