Pseudomonas aeruginosa Flashcards

1
Q

most important species in pseudomonas genus

A

pseudomonas aeruginosa

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2
Q

respiration
gram
shape
motility
reservoir
opportunistic?
oxidase
catalase

A

aerobic
gram negative
rod
motile – polar flagella
reservoir is environment
opportunistic – detected in healthy animals, found in environment
oxidase positive
catalase positive

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3
Q

odor, and why

A

fruity grape like odor
aminoacetophenone

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4
Q

pigments

A

pyocyanin

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5
Q

multidrug resistance

A

impermeability
multidrug efflux pumps
aminoglycoside modifying enzymes
biofilm – alginate
beta lactamases – resistant to penicillin

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6
Q

virulence

A

exotoxin A
phospholipase C – hemolysin
pyocyanin – ROS
pyoverdin – siderophores
degradative enzymes
pili
flagella
LPS
biofilm – mucoid EPS, alginate
T3SS – exoenzyme S

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7
Q

what location does it prefer

A

body sites exposed to oxygen
skin, ears, respiratory tract

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8
Q

skin infections

A

fleece rot – sheep
pyoderma – dogs

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9
Q

ear infections

A

otitis externa – dogs, cats
otitis media/interna – chinchillas

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10
Q

eye infections

A

ulcerative keratitis – dogs, cats, horses

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11
Q

respiratory infections

A

pneumonia – dogs, cats, chinchillas

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12
Q

bloodstream infections

A

bacteremia, septicemia – chinchillas

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13
Q

other infections

A

mastitis – cattle, sheep
necrotic stomatitis (oral cavity infection) – snakes

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14
Q

nutrient agar

A

fruity grape odor
pyocyanin pigment
smooth round colonies

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15
Q

macconkey agar

A

non lactose fermenter
white colorless colonies

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16
Q

blood agar

A

beta hemolysis (hemolysin) with a clear zone

17
Q

treatment

A

eliminate source of infection
antibiotics difficult – multidrug resistance
antimicrobial susceptibility testing
vaccines for farmed mink and chinchillas