Enterobacteriaceae

Question 1

gram
shape
catalase
oxidase

Answer 1

gram negative
rod
catalase positive
oxidase negative

Question 2

what do they hide cell wall with

Answer 2

outer membrane

Question 3

CEEK

Answer 3

citrobacter
e coli
enterobacter
klebsiella

Question 4

CEEK lactose?

Answer 4

lactose fermenters
coliform bacteria

Question 5

SYP-Sh

Answer 5

salmonella
proteus
yersina
shigella

Question 6

SYP-Sh lactose?

Answer 6

lactose non fermenters
highly pathogenic

Question 7

IMViC tests

Answer 7

indole
methylred
VP
citrate

Question 8

macconkey negatives

Answer 8

salmonella, yersinia, proteus
yellow color

Question 9

macconkey positives

Answer 9

citrobacter, enterobacter, klebsiella
red color

Question 10

indole negatives

Answer 10

klebsiella, enterobacter, salmonella, yersinia, proteus

Question 11

indole positives

Answer 11

citrobacter

Question 12

voges proskauer negatives

Answer 12

citrobacter, salmonella, yersinia, proteus

Question 13

voges proskauer positives

Answer 13

klebsiella, enterobacter

Question 14

citrate negatives

Answer 14

yersinia, proteus

Question 15

citrate positives

Answer 15

salmonella, klebsiella, enterobacter, citrobacter

Question 16

urease negatives

Answer 16

salmonella, enterobacter

Question 17

urease positives

Answer 17

yersinia, proteus, klebsiella, citroabcter

Question 18

TSI CO2 gas, yellow

Answer 18

enterobacter, klebsiella

Question 19

TSI H2S gas, black

Answer 19

salmonella, proteus, citrobacter

Question 20

TSI no gas, yellow

Answer 20

yersinia

Question 21

respiration
fastidious?
spore?
media?
motility?

Answer 21

facultative anaerobes
non fastidious
non spore forming
grow on bile media (Macconkey)
most are motile (except shigella and klebsiella)

Question 22

general habitat
Proetus habitat

Answer 22

small and large intestines
proteus – lungs, vulva, vagina

Question 23

transmission route
yersinia pestis transmission
opportunistic?
commensal?

Answer 23

oral fecal transmission route
yersinia pestis – flea bites
commensal of GI tract, opportunistic pathogen

Question 24

exit

Answer 24

feces, urine, milk, nasal discharge

Question 25

what do they cause

Answer 25

GI infection -- diarrhea, dehydration lever abscess UTI meningitis mastitis

Question 26

what about diarrhea causes morbidity and mortality

Answer 26

acid base imbalance electrolyte imbalance

Question 27

surface structures

Answer 27

capsule flagella fimbriae adhesin invasins LPS -- pyrogenic, fever

Question 28

enzymes

Answer 28

catalase superoxide disumutase -- detoxify free radicals

Question 29

toxins

Answer 29

hemolysin -- kills host RBC, make iron available siderophores, enterobactin -- rob irone from host cells pathogenicity island -- encodes toxins, enzymes, T3SS

Question 30

antigenic diversity

Answer 30

LPS (O) antigen flagella (H antigen) capsule (K antigen)

Question 31

what does lipid A do

Answer 31

endotoxin destroys blood vessels, induce fever

Question 32

culture media

Answer 32

blood agar macconkey eosin methylene blue (EMB) triple sugar iron

Question 33

contributes 3 members of ESKAPE

Answer 33

enterobacter aerogenes (klebsiella aerogenes) klebsiella pnuemoniae enterobacter cloacae