Enterobacteriaceae Flashcards
gram
shape
catalase
oxidase
gram negative
rod
catalase positive
oxidase negative
what do they hide cell wall with
outer membrane
CEEK
citrobacter
e coli
enterobacter
klebsiella
CEEK lactose?
lactose fermenters
coliform bacteria
SYP-Sh
salmonella
proteus
yersina
shigella
SYP-Sh lactose?
lactose non fermenters
highly pathogenic
IMViC tests
indole
methylred
VP
citrate
macconkey negatives
salmonella, yersinia, proteus
yellow color
macconkey positives
citrobacter, enterobacter, klebsiella
red color
indole negatives
klebsiella, enterobacter, salmonella, yersinia, proteus
indole positives
citrobacter
voges proskauer negatives
citrobacter, salmonella, yersinia, proteus
voges proskauer positives
klebsiella, enterobacter
citrate negatives
yersinia, proteus
citrate positives
salmonella, klebsiella, enterobacter, citrobacter
urease negatives
salmonella, enterobacter
urease positives
yersinia, proteus, klebsiella, citroabcter
TSI CO2 gas, yellow
enterobacter, klebsiella
TSI H2S gas, black
salmonella, proteus, citrobacter
TSI no gas, yellow
yersinia
respiration
fastidious?
spore?
media?
motility?
facultative anaerobes
non fastidious
non spore forming
grow on bile media (Macconkey)
most are motile (except shigella and klebsiella)
general habitat
Proetus habitat
small and large intestines
proteus – lungs, vulva, vagina
transmission route
yersinia pestis transmission
opportunistic?
commensal?
oral fecal transmission route
yersinia pestis – flea bites
commensal of GI tract, opportunistic pathogen
exit
feces, urine, milk, nasal discharge
what do they cause
GI infection – diarrhea, dehydration
lever abscess
UTI
meningitis
mastitis
what about diarrhea causes morbidity and mortality
acid base imbalance
electrolyte imbalance
surface structures
capsule
flagella
fimbriae
adhesin
invasins
LPS – pyrogenic, fever
enzymes
catalase
superoxide disumutase – detoxify free radicals
toxins
hemolysin – kills host RBC, make iron available
siderophores, enterobactin – rob irone from host cells
pathogenicity island – encodes toxins, enzymes, T3SS
antigenic diversity
LPS (O) antigen
flagella (H antigen)
capsule (K antigen)
what does lipid A do
endotoxin
destroys blood vessels, induce fever
culture media
blood agar
macconkey
eosin methylene blue (EMB)
triple sugar iron
contributes 3 members of ESKAPE
enterobacter aerogenes (klebsiella aerogenes)
klebsiella pnuemoniae
enterobacter cloacae