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Escherichia coli Flashcards

1
Q

gram
shape
respiration
motility?
ferment glucose?
ferment lactose?
oxidase
catalase

A

gram negative
rod
facultative anaerobe
motile by peritrichous flagella
ferment glucose
ferment lactose
oxidase negative
catalase positive

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2
Q

where do they normally live, are most pathogenic

A

normally live in intestinal tract
most are avirulent

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3
Q

how can they cause disease

A

when acquire virulence
escape gut

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4
Q

antimicrobial resistane casues what and is due to what

A

causes treatment failure
due to beta lactams, fluorquinolones

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5
Q

what is LPS lipid A

A

active component of endotoxin

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6
Q

which ones are in small bowel

A

ETEC
EPEC

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7
Q

which ones are in large bowel

A

EHEC
EIEC

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8
Q

what is ETEC, where is it located

A

enterotoxigenic e coli
small bowel

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9
Q

ETEC fimbrial adhesins

A

attachment to small intestine

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10
Q

ETEC enterotoxins

A

heat labile enterotoxin (LT) – cAMP
heat stable enterotoxin (ST) – cGMP accumulation in cells

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11
Q

what type of diarrhea does ETEC cauase

A

watery diarrhea

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12
Q

ETEC – neonatal diarrhea – animals affected

A

enteric colibacillosis
newborn calves, lambs, piglets (first week of life)

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13
Q

ETEC – neonatal diarrhea – how do animals become resistant

A

shed fibroreceptors as age – become resistant to ETEC

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14
Q

ETEC – neonatal diarrhea – syptoms

A

diarrhea, dehydration, death

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15
Q

ETEC – neonatal diarrhea – treatment

A

milk with fluids and electrolytes
hydration

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16
Q

ETEC – neonatal diarrhea – control

A

feed ample colostrum

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17
Q

ETEC – postweaning diarrhea – animals affected

A

enteric colibacillosis
common cause of death in weaned pigs (1-2 weeks post weaning)

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18
Q

ETEC – postweaning diarrhea – what can case it

A

ETEC or EPEC

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19
Q

ETEC – postweaning diarrhea – symptoms

A

watery diarrhea, loss of appetite, purplish discoloration of skin

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20
Q

ETEC – postweaning diarrhea – control

A

vaccination, breeding for disease ressitance

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21
Q

ETEC – postweaning diarrhea – treatment

A

antimicrobial resistance testing important

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22
Q

what is EPEC and where is it located

A

enteropathogenic e coli
small bowel

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23
Q

EPEC – adhesion

A

no fimbrio adhesion
intimin

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24
Q

EPEC – lesions

A

pedestal formation – loss of microvilli
attaching and effacing lesions

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25
EPEC -- symptoms
watery diarrhea contributes to post weaning diarrhea
26
what is EHEC and where is it located
enterohemorrhagic e coli large bowel
27
virulence of EHEC
intimin T3SS attaching and effacing lesions
28
EHEC key virulence factor
shiga toxin -- hemorrhagic diarrhea, kidney failure
29
other names for EHEC
shiga toxin producing e coli (STEC) verocytotoxin producing e coli (VTEC)
30
primary reservoir of EHEC, do they get sick
cattle cattle remain healthy
31
why do cattle remain healthy with EHEC
cattle don't have shiga toxin receptor Gb3
32
EHEC -- edema disease of pigs -- age of pigs, what type of shiga toxin
1-2 weeks after weaning shiga toxin strain stx2e
33
EHEC -- edema disease of pigs -- symptoms
swelling of eyelids, muscular tremors, unusual squeal or snort, neurological dysfunction, flaccid paralysis and death within 36 hours
34
what is EIEC and location
enteroinvasive e coli large bowel
35
what type of pathogen is EIEC
obligate intracellular pathogen in colon
36
EIEC virulence
ipaH T3SS
37
EIEC symptoms
inflammation, necrosis, ucleration of bowel watery to bloody diarrhea with fever
38
which speceis relatively resistnat to EIEC
dogs and cats relatively resistant human enteric pathogen
39
shigella: gram shape motility? toxin symptoms disease hosts
gram negative rod non motile, no flagella produces shiga toxin bloody diarrhea shigellosis more of a human pathogen can invade host cell
40
what is ExPEC, different types
extraintestinal pathigenic e coli SEPEC APEC UPEC MPEC
41
what is SEPEC animals affected what does it invade
septicemia e coli newborn calves, lambs, poultry, immunocompromised animals invasion of bloodstream -- systemic infection
42
SEPEC -- virulence
fimbrial ahdesions serum resistance aerobactin iron uptake LPS cytotoxic necrotizing factors
43
SEPEC in lambs
watery mouth disease in lambs death
44
SEPEC diseases
pyrexia depression weakness hypothermia prostration meningitis pneumonia arthritis diarrhea
45
what is APEC animals affected and type of disease
avian pathogenic e coli systemic disease of birds and poultry
46
APEC virulence
type I (FI) and P fimbriae LPS serum resistance aerobactin iron uptake
47
APEC diseases
septicemia egg yolk peritonitis penumonia airsacculitis salpingitis
48
what is UPEC zoonosis? what does it cause
uropathogenic e coli zoonotic potential UTI -- cystitis, pyelonephritis
49
UPEC virulence
type I pili attachment hemolysin A LPS CNF-1 -- inflammaotry response aerobactin, capsule -- survival in blood intracellular bacterial communities quiescent intracellular reservoirs
50
UPEC -- males or females more susceptible, why
females more susceptible bacteria enter through urethra males have long urethra -- bacteria don't reach bladder as easily
51
UPEC diseases
cystitis, pyelonephritis, pyometra, prostatitis
52
what is MPEC what does it cause how is it caused
mammary pathogenic e coli casues mastitis casued by fecal contamiantion of mammary gland through teat sphincter relaxation
53
MPEC symptoms
udder swelling, pain, and redness reduced milk production and quality
54
MPEC treatment
mild mastitis cured without treatment when systemic -- requires treatment
55
blood agar macconkey agar EMB chromogenic agar
hemolytic on blood agar pink colonies on macconkey metallic sheen on EMB dark blue on chromogenic agar
56
IVMiC test
indole positive methyl red positive voges proskauer negative citrate negative
57
molecular typing
MLST PFGE RAPD
58
identification
ETEC -- ELISA, PCR, latex agglutination EPEC -- urease +, PCR, effacement of mucosal surface STEC -- edema, bloody diarrhea, hemolytic on blood agar, serotype SEPEC -- isolation from blood, PCR (serum resistance) UPEC -- isolation from urine, type I pili
59
treatment
rehydration antibiotics -- test for susceptibility
60
control
colostrum after birth reduced stress at weaning hygiene vaccines
61
ETEC summary
intestinal e coli fimbriae, enterotoxins newborn calves, lambs, piglets watery diarrhea neonatal diarrhea, post weaning diarrhea
62
EPEC summary
intestinal e coli intimin, T3SS newborn calves, lambs, pigments watery diarrhea post weaning diarrhea
63
EHEC (STEC/VTEC) summary
intestinal e coli intimin. T3SS, shiga toxin bloody diarrhea edema disease
64
EIEC (shigella) sumamry
intestinal e coli invasion humans bloody diarrhea, dysentery
65
ExPEC summary
extraintestinal e coli serum resistance, aerobactin, type I and P fimbriae, hemolysin, CNF1 newborn calves, lambs, piglets, poultry, dogs, cows, sows septicemia, systemic infection colisepticemia, UTI, mastitis

B&M Exam 2 (31 decks)

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