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B&M Exam 2 > Escherichia coli > Flashcards

Escherichia coli Flashcards

1
Q

gram
shape
respiration
motility?
ferment glucose?
ferment lactose?
oxidase
catalase

A

gram negative
rod
facultative anaerobe
motile by peritrichous flagella
ferment glucose
ferment lactose
oxidase negative
catalase positive

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2
Q

where do they normally live, are most pathogenic

A

normally live in intestinal tract
most are avirulent

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3
Q

how can they cause disease

A

when acquire virulence
escape gut

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4
Q

antimicrobial resistane casues what and is due to what

A

causes treatment failure
due to beta lactams, fluorquinolones

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5
Q

what is LPS lipid A

A

active component of endotoxin

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6
Q

which ones are in small bowel

A

ETEC
EPEC

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7
Q

which ones are in large bowel

A

EHEC
EIEC

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8
Q

what is ETEC, where is it located

A

enterotoxigenic e coli
small bowel

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9
Q

ETEC fimbrial adhesins

A

attachment to small intestine

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10
Q

ETEC enterotoxins

A

heat labile enterotoxin (LT) – cAMP
heat stable enterotoxin (ST) – cGMP accumulation in cells

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11
Q

what type of diarrhea does ETEC cauase

A

watery diarrhea

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12
Q

ETEC – neonatal diarrhea – animals affected

A

enteric colibacillosis
newborn calves, lambs, piglets (first week of life)

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13
Q

ETEC – neonatal diarrhea – how do animals become resistant

A

shed fibroreceptors as age – become resistant to ETEC

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14
Q

ETEC – neonatal diarrhea – syptoms

A

diarrhea, dehydration, death

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15
Q

ETEC – neonatal diarrhea – treatment

A

milk with fluids and electrolytes
hydration

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16
Q

ETEC – neonatal diarrhea – control

A

feed ample colostrum

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17
Q

ETEC – postweaning diarrhea – animals affected

A

enteric colibacillosis
common cause of death in weaned pigs (1-2 weeks post weaning)

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18
Q

ETEC – postweaning diarrhea – what can case it

A

ETEC or EPEC

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19
Q

ETEC – postweaning diarrhea – symptoms

A

watery diarrhea, loss of appetite, purplish discoloration of skin

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20
Q

ETEC – postweaning diarrhea – control

A

vaccination, breeding for disease ressitance

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21
Q

ETEC – postweaning diarrhea – treatment

A

antimicrobial resistance testing important

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22
Q

what is EPEC and where is it located

A

enteropathogenic e coli
small bowel

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23
Q

EPEC – adhesion

A

no fimbrio adhesion
intimin

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24
Q

EPEC – lesions

A

pedestal formation – loss of microvilli
attaching and effacing lesions

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25
Q

EPEC – symptoms

A

watery diarrhea
contributes to post weaning diarrhea

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26
Q

what is EHEC and where is it located

A

enterohemorrhagic e coli
large bowel

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27
Q

virulence of EHEC

A

intimin
T3SS
attaching and effacing lesions

28
Q

EHEC key virulence factor

A

shiga toxin – hemorrhagic diarrhea, kidney failure

29
Q

other names for EHEC

A

shiga toxin producing e coli (STEC)
verocytotoxin producing e coli (VTEC)

30
Q

primary reservoir of EHEC, do they get sick

A

cattle
cattle remain healthy

31
Q

why do cattle remain healthy with EHEC

A

cattle don’t have shiga toxin receptor Gb3

32
Q

EHEC – edema disease of pigs – age of pigs, what type of shiga toxin

A

1-2 weeks after weaning
shiga toxin strain stx2e

33
Q

EHEC – edema disease of pigs – symptoms

A

swelling of eyelids, muscular tremors, unusual squeal or snort, neurological dysfunction, flaccid paralysis and death within 36 hours

34
Q

what is EIEC and location

A

enteroinvasive e coli
large bowel

35
Q

what type of pathogen is EIEC

A

obligate intracellular pathogen in colon

36
Q

EIEC virulence

A

ipaH
T3SS

37
Q

EIEC symptoms

A

inflammation, necrosis, ucleration of bowel
watery to bloody diarrhea with fever

38
Q

which speceis relatively resistnat to EIEC

A

dogs and cats relatively resistant
human enteric pathogen

39
Q

shigella:
gram
shape
motility?
toxin
symptoms
disease
hosts

A

gram negative
rod
non motile, no flagella
produces shiga toxin
bloody diarrhea
shigellosis
more of a human pathogen
can invade host cell

40
Q

what is ExPEC, different types

A

extraintestinal pathigenic e coli
SEPEC
APEC
UPEC
MPEC

41
Q

what is SEPEC
animals affected
what does it invade

A

septicemia e coli
newborn calves, lambs, poultry, immunocompromised animals
invasion of bloodstream – systemic infection

42
Q

SEPEC – virulence

A

fimbrial ahdesions
serum resistance
aerobactin iron uptake
LPS
cytotoxic necrotizing factors

43
Q

SEPEC in lambs

A

watery mouth disease in lambs
death

44
Q

SEPEC diseases

A

pyrexia
depression
weakness
hypothermia
prostration
meningitis
pneumonia
arthritis
diarrhea

45
Q

what is APEC
animals affected and type of disease

A

avian pathogenic e coli
systemic disease of birds and poultry

46
Q

APEC virulence

A

type I (FI) and P fimbriae
LPS
serum resistance
aerobactin iron uptake

47
Q

APEC diseases

A

septicemia
egg yolk peritonitis
penumonia
airsacculitis
salpingitis

48
Q

what is UPEC
zoonosis?
what does it cause

A

uropathogenic e coli
zoonotic potential
UTI – cystitis, pyelonephritis

49
Q

UPEC virulence

A

type I pili attachment
hemolysin A
LPS
CNF-1 – inflammaotry response
aerobactin, capsule – survival in blood
intracellular bacterial communities
quiescent intracellular reservoirs

50
Q

UPEC – males or females more susceptible, why

A

females more susceptible
bacteria enter through urethra
males have long urethra – bacteria don’t reach bladder as easily

51
Q

UPEC diseases

A

cystitis, pyelonephritis, pyometra, prostatitis

52
Q

what is MPEC
what does it cause
how is it caused

A

mammary pathogenic e coli
casues mastitis
casued by fecal contamiantion of mammary gland through teat sphincter relaxation

53
Q

MPEC symptoms

A

udder swelling, pain, and redness
reduced milk production and quality

54
Q

MPEC treatment

A

mild mastitis cured without treatment
when systemic – requires treatment

55
Q

blood agar
macconkey agar
EMB
chromogenic agar

A

hemolytic on blood agar
pink colonies on macconkey
metallic sheen on EMB
dark blue on chromogenic agar

56
Q

IVMiC test

A

indole positive
methyl red positive
voges proskauer negative
citrate negative

57
Q

molecular typing

A

MLST
PFGE
RAPD

58
Q

identification

A

ETEC – ELISA, PCR, latex agglutination
EPEC – urease +, PCR, effacement of mucosal surface
STEC – edema, bloody diarrhea, hemolytic on blood agar, serotype
SEPEC – isolation from blood, PCR (serum resistance)
UPEC – isolation from urine, type I pili

59
Q

treatment

A

rehydration
antibiotics – test for susceptibility

60
Q

control

A

colostrum after birth
reduced stress at weaning
hygiene
vaccines

61
Q

ETEC summary

A

intestinal e coli
fimbriae, enterotoxins
newborn calves, lambs, piglets
watery diarrhea
neonatal diarrhea, post weaning diarrhea

62
Q

EPEC summary

A

intestinal e coli
intimin, T3SS
newborn calves, lambs, pigments
watery diarrhea
post weaning diarrhea

63
Q

EHEC (STEC/VTEC) summary

A

intestinal e coli
intimin. T3SS, shiga toxin
bloody diarrhea
edema disease

64
Q

EIEC (shigella) sumamry

A

intestinal e coli
invasion
humans
bloody diarrhea, dysentery

65
Q

ExPEC summary

A

extraintestinal e coli
serum resistance, aerobactin, type I and P fimbriae, hemolysin, CNF1
newborn calves, lambs, piglets, poultry, dogs, cows, sows
septicemia, systemic infection
colisepticemia, UTI, mastitis

B&M Exam 2 (31 decks)

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