Edwardsiella tarda Flashcards
respiration
gram
shape
motility
opportunistic?
zoonotic?
intracellular?
oxidase
catalase
facultative anaerobe
gram negative
rod
motile by peritrichous flagella
opportunistic pathogen
zoonotic potential – foodborne and waterborne infections
intracellular pathogen
oxidase negative
catalase positive
what family is it a member of
enterobacteriaceae
habitat
freshwater
fish and aquatic enviornments
virulence
flagella
LPS
fimbriae
hemolysin
proteinase
T3SS
T6SS
serum resistance
capsule
biofilm – quorum sensing (increased bacterial denisty)
5 minutes post infection
bacteria biond to host cells
30 minutes post infection
bacteria found in vacuole in cytoplasm
3.5 hours post infection
multiple bacteria in cytoplasm – intracellular replication
4.5 hours post infection
plasma membrane destroyed – escape from cell
edwardsiellosis in fish – species and hosts
flounder – e tarda, e piscicida
channel catfish, tilapia – e ictaluri
eel – e anguillarum
edwardsiellosis in fish – lesions
granuloma in multiple organs
ascites
skin ulcerations
hemorrhagic septicemia
human disease
diarrhea
rarely bacteremia and septicemia
transmission
ingestion of contaminated water
typhlocolitis post mortem findings
greenish fluid, fecal material, deposition of fibrin in abdomen
ruptures in cecum, petechia in colon, necrosis in cecum
diagnosis
isolation and identification
agglutination test, ELISA
blood agar
macconkey
brilliant green agar
XLD agar
hemolytic on blood agar
pale colorless on macconkey
no growth on brilliant green agar – selective for salmonella
red, black center on XLD agar