Edwardsiella tarda Flashcards

1
Q

respiration
gram
shape
motility
opportunistic?
zoonotic?
intracellular?
oxidase
catalase

A

facultative anaerobe
gram negative
rod
motile by peritrichous flagella
opportunistic pathogen
zoonotic potential – foodborne and waterborne infections
intracellular pathogen
oxidase negative
catalase positive

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2
Q

what family is it a member of

A

enterobacteriaceae

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3
Q

habitat

A

freshwater
fish and aquatic enviornments

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4
Q

virulence

A

flagella
LPS
fimbriae
hemolysin
proteinase
T3SS
T6SS
serum resistance
capsule
biofilm – quorum sensing (increased bacterial denisty)

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5
Q

5 minutes post infection

A

bacteria biond to host cells

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6
Q

30 minutes post infection

A

bacteria found in vacuole in cytoplasm

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7
Q

3.5 hours post infection

A

multiple bacteria in cytoplasm – intracellular replication

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8
Q

4.5 hours post infection

A

plasma membrane destroyed – escape from cell

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9
Q

edwardsiellosis in fish – species and hosts

A

flounder – e tarda, e piscicida
channel catfish, tilapia – e ictaluri
eel – e anguillarum

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10
Q

edwardsiellosis in fish – lesions

A

granuloma in multiple organs
ascites
skin ulcerations
hemorrhagic septicemia

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11
Q

human disease

A

diarrhea
rarely bacteremia and septicemia

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12
Q

transmission

A

ingestion of contaminated water

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13
Q

typhlocolitis post mortem findings

A

greenish fluid, fecal material, deposition of fibrin in abdomen
ruptures in cecum, petechia in colon, necrosis in cecum

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14
Q

diagnosis

A

isolation and identification
agglutination test, ELISA

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15
Q

blood agar
macconkey
brilliant green agar
XLD agar

A

hemolytic on blood agar
pale colorless on macconkey
no growth on brilliant green agar – selective for salmonella
red, black center on XLD agar

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16
Q

treatment

A

antibiotics
rehydration

17
Q

control

A

quarantine
water quality