Radio Immunoassay Flashcards
First Immunoassay to be developed
Radioimmunoassay
In radioimmunoassay what is being used for labelling
Radioisotopes
What are the radioisotopes that are used
125 I
131 I
3 H
Most commonly used radioisotopes and has 60 days half life
125 I
Instrument used to measure radioactivity
Scintillation counter
Crystal Scintillation counter
Gamma
Liquid scintillation counter
Beta
Disadvatange of using radioimmunoassay
Health Hazard
Disposal Problem
Require the use of Expensive Equipment
Short shelf life
Application used for measuring Total IgE
RIST
RadioImmunoAbsorbent Test
Application used for measuring Allergen-specific IgE
RAST
RadioAllergoAbsorbent Test
Radioimmunoassay by which Labeled Antigen competes with Unlabeled antigen of the patient to the limited number of binding sites
Competitive Binding Assays
Radioimmunoassay by which analyte to be measured is SANDWICHED BETWEEN 2 ANTIBODIES
Noncompetitive ImmunoRadioMetric Assay (IRMA)