Polymerase Chain Reaction

Question 1

In vitro Replication of DNA; Amplification of DNA

Answer 1

PCR
Polymerase Chain Reaction

Question 2

Denaturation happens under what temperature

Answer 2

92 deg cel

Question 3

dsDNA is cleaved to form single strand of DNA

Answer 3

Denaturation

Question 4

Annealing happens under what temperature

Answer 4

52 deg cel

Question 5

Process of PCR that allows the single DNA strand to cool down for the attachment of the Primer’s

Answer 5

Annealing

Question 6

Elongation happens at what temperature

Answer 6

72 deg celsius

Question 7

It is the process by which heat-stable DNA polymerase (Taq polymerase) is attached to the 3’ end of the primer

Answer 7

Elongation

Question 8

piece of genetic material formed in the natural event or artificial amplification

Answer 8

Amplicon

Question 9

Components of DNA for PCR

Answer 9

1. Template DNA
2. Deoxyribonucleotide
3. Oligonucleotides Primer
4. DNA polymerase
5. Buffer for Optimal pH

Question 10

• Single-stranded DNA
• Complementary for 3’ end
• template for New DNA

Answer 10

Primer

Question 11

Primer consist of _____ bases in length

Answer 11

18-30 bases

Question 12

Serves as the landmark for PCR

Answer 12

Oligonucleotide primer

Question 13

• RNA-dependent DNA polymerase
• Converts RNA to DNA
• Used for the detection of RNA viruses

Answer 13

Reverse Transcriptase PCR

Question 14

• Originally called Real-time PCR
• Uses Fluorescence-resonance Energy Transfer
• results can be seen at the end of each cycle

Answer 14

Quantitative PCR

Question 15

thermo-stable polymerase

Answer 15

Taq polymerase

Question 16

Taq polymerase is retrieved from

Answer 16

Thermus aquaticus