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SEROLOGY > II: Syphilis > Flashcards

II: Syphilis Flashcards

1
Q

Non Treponemal Test

Principle: Quantitative and Qualitative Flocculation Test

A

VDRL
Venereal Disease Research Laboratory Test

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2
Q

VDRL Specimen

A

50 ul of Serum/CSF

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3
Q

Specimen in VDRL must be heated for ____ under ____

A

30 mins
56 deg Cel

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4
Q

VDRL

Antigen consist of

A

0.03% Cardiolipin
0.9% Cholesterol
0.21% Lecithin

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5
Q

Antigen delivery needle required for VDRL

A

Hamilton Syringe

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6
Q

VDRL Antigen Delivery Needle:

Qualitative

A

18 g
60 drops Ag suspension/mL

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7
Q

VDRL Antigen Delivery Needle:

Quantitative

A

19 g
75 drops Ag suspension / mL

23 g
100 drops saline / mL

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8
Q

VDRL Antigen Delivery Needle:

CSF

A

21 or 22 g
100 drops / mL

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9
Q

VDRL Rotator:

Serum

A

4 mins
180 rpm

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10
Q

VDRL Rotator:

CSF

A

8 mins
180 rpm

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11
Q

VDRL Reading of Reports under:

A

LPO (100x)

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12
Q

VDRL Reporting:

Nonreactive

A

No clumping / Slight roughness

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13
Q

VDRL Reporting:

Weakly Reactive

A

small clumps

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14
Q

VDRL Reporting:

Reactive

A

Medium-large clumps

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15
Q

VDRL:

Weakly reactive or reactive result must be subject to

A

Quantitative result (dilution from 1:2 to 1:32)

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16
Q

VDRL:

A positive test on a spinal fluid is a diagnostic of

A

Neurosyphilis

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17
Q

Non Treponemal Test that is a modified VDRL with CHARCOAL PARTICLES

A

Rapid Plasma Reagin (RPR) Test

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18
Q

RPR Test Specimen

A

50 uL serum (NO HEATING REQUIREMENT)

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19
Q

RPR Antigen consist of:

A

Same with VDRL Reagent with addition of

EDTA
Charcoal
Thimerosal
Choline chloride

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20
Q

RPR:

Purpose of Charcoal

A

Enhance macroscopic visualization of reaction

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21
Q

RPR:

Purpose of thimerosal

A

Preservative

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22
Q

RPR:

Purpose of Choline Chloride

A

Inactive complement

23
Q

RPR:

Rotator Requiremen

A

8 mins
100 rpm

24
Q

RPR:

Antigen Delivery Needle

A

20 g
60 drops Ag suspension / mL

25
Q

RPR:

Non Reactive

A

no clumping / slight roughness

26
Q

RPR:

Reactive

27
Q

Treponemal Test of Reference

  • If individual is reactive; it persist to life
A

Treponemal Pallidum Immobilization Test (TPI)

28
Q

Treponemal Test:

TPI

Treponemal Antibody in the presence of complement will inhibit the normal movement of active treponemes acquired from

A

Testicular Chancre of Rabbit

29
Q

TREPONEMAL TEST:

Patient serum in Fluorescent Treponemal Antibody Absorption Test must be

A

Heat Inactivated

30
Q

TREPONEMAL TEST:

In Fluorescent Treponemal Antibody Absorption Test it is added to avoid cross-reactivity with other treponemes other than T. pallidum

A

Nonpathogenic Treponemes strains (REITER STRAIN)

31
Q

TREPONEMAL TEST:

Fluorescent Treponemal Antibody Absorption Test

Strain that is added on the slide

A

Nichols strain

32
Q

REPONEMAL TEST:

Fluorescent Treponemal Antibody Absorption Test

Result must be observed using

A

Fluorescence Microscope

33
Q

REPONEMAL TEST:

Fluorescent Treponemal Antibody Absorption Test

Reaction is equivalent to the

A

Intensity of Green Fluorescence

34
Q

REPONEMAL TEST:

Fluorescent Treponemal Antibody Absorption Test

Reactive

A

2+ green fluorescence

35
Q

TREPONEMAL TEST:

Fluorescent Treponemal Antibody Absorption Test

Negative

A

No Fluorescence

36
Q

TREPONEMAL TEST:

Fluorescent Treponemal Antibody Absorption Test

1+

A

Repeat test with 2nd specimen after 1 to 2 weeks

37
Q

TREPONEMAL TEST:
Agglutination Test (4)

A
  1. Hemagglutination Treponemal Test for Syphilis (HATTS)
  2. Treponema Pallidum Hemagglutination Assay (TPHA)
  3. MicroHemagglutination Assay for Antibodies of T. pallidum (MHA-TPA)
  4. T. pallidum Particle Agglutination (TPPA)
38
Q

TREPONEMAL TEST:
Agglutination Test

Uses gluteraldehyde-stabilized TURKEY RBC

39
Q

TREPONEMAL TEST:
Agglutination Test

Uses Tanned Sheep’s RBC coated with antigen of Nichol’s strain

40
Q

TREPONEMAL TEST:
Agglutination Test

Same with TPHA but uses Microtiter Plates

w/

Formalinized Sheep’s RBC
coated w/ nichol’s strain

41
Q

TREPONEMAL TEST:
Agglutination Test

Uses Gelatin Particles

42
Q

Other Treponema-Associated Diseases in Humans

T. pertenue

A

YAWS

“pertenYOWS”

43
Q

Other Treponema-Associated Diseases in Humans

T. carateum

A

Pinta

“pinta-cara”

44
Q

Other Treponema-Associated Diseases in Human

T. pallidum endemicum

A

Bejel
Endemic Syphilis

45
Q

Other Treponema-Associated Diseases in Humans

T. cuniculi (Wasserman NR)

A

Rabbit’s Syphilis

46
Q

VDRL uses slides with

A

Ceramic rings

47
Q

VDRL

Purpose of Cardiolipin

A

Main reacting component

48
Q

VDRL

Purpose of Cholesterol

A

Enhances reacting surface of cardiolipin

49
Q

VDRL

Purpose of Lecithin

A

removes anti-complement activity of cardiolipin

50
Q

NONTREPONEMAL TEST (6)

A
  1. VDRL
  2. RPR
  3. Plasmacrit
  4. Toluidine Red Unheated Serum Test (TRUST)
  5. Unheated Serum Reagin (USR)
  6. Reagin SCREEN test (RST)
51
Q

TREPONEMAL TEST (7)

A
  1. T. pallidum Immobilization Test (TPI)
  2. Fluorescent Treponemal Antibody Test
  3. Fluorescent Treponemal Antibody Absorption Test
  4. T.pallidum Hemagglutination
  5. T. pallidum complement fixation
  6. Reiter’s protein complement fixation
  7. Direct Fluorescent Antibody Test
52
Q

Used for diagnosing Congenital Syphilis & Neurosyphilis

53
Q

What substance is detected by VDRL and RPR test

A

Anticardiolipin Antibody

54
Q

Establish diagnosis for Late Latent Syphilis

A

Treponemal Test

SEROLOGY (17 decks)

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