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SEROLOGY > Precipitation > Flashcards

Precipitation Flashcards

1
Q

combination of SOLUBLE ANTIGENS and SOLUBLE ANTIBODIES to form insoluble complexes

A

Precipitation

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2
Q

Optimum precipitation occurs; number of multivalent sites of antigen and antibody are EQUAL

A

Zone of Equivalence

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3
Q

Presence of Excess Antibodies is called

A

Prozone

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4
Q

Remedy for Prozone phenomenon

A

Serum dilution

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5
Q

Presence of excess Antigen is called

A

Postzone

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6
Q

Remedy for Postzone phenomenon

A

Repeat test after a week

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7
Q

Prozone and Postzone phenomenon may lead to False ___ result

A

False negative

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8
Q

Measurement of Precipitation in Fluid Medium (2)

A

Turbidimetry
Nepholometry

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9
Q

Measurement of Precipitation in Fluid Medium

Has a principle of measuring light by BLOCKED LIGHT at a DIRECT LINE

A

Turbidimetry

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10
Q

Measurement of Precipitation in Fluid Medium

Has a principle of measuring light by SCATTERED LIGHT at AN ANGLE

A

Nephelometry

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11
Q

Which of the measurement of Precipitation in fluid medium is more sensitive

A

Turbidimetry (readings are taken before point of equivalence is reached)

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12
Q

Process of measuring precipitation without the used of electric current;

USES agar, gel, agarose

A

Passive immunodiffusion

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13
Q

Passive immunodiffusion:

Antibody is placed on the agarose
Antigen is placed on top, moves down the gels, and precipitation occurs

A

Single LINEAR Diffusion (Oudin)

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14
Q

Passive immmunodiffusion:

In Single Linear Diffusion the Antigen Concentration is based on the

A

Precipitation that moves down

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15
Q

A passive immunodiffusion by which the:

Antibody is uniformly distributed in the support gel

Antigen is placed a well cut into the gell

Area of rings is measured and is equal to Antigen Concentration

A

Single Radial Diffusion/ Radial Immunodiffusion

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16
Q

Single Radial Diffusion:

Antigen is diffused; Equivalence is reached and no further changes in diameter

A

Mancini / Endpoint Method

17
Q

Single Radial Diffusion:

Mancini/Endpoint Method antigen is measured by

A

Square of the Diameter

18
Q

Single Radial Diffusion:

Measurement of the precipitation is made before the point of equivalence has been reached; only occurs about 18 hrs / 24 hrs

A

Fahey / McKelvey / Kinetic Method

19
Q

Single Radial Diffusion:

In Fahey /McKelvey Method the amount of precipitation if measured by the

A

Diameter by LOG OF ANTIGEN CONCENTRATION

20
Q

A Passive immunodiffusion:

Allows diffusion of Antigen and antibody through semi solid medium in 2 dimensions

A

Double Radial Diffusion (Ouchterlony)

21
Q

A passive immunodiffusion:

Antibody is placed on the central well

Antigen is placed on the surrounding

A

Double Radial Diffusion

22
Q

In Double Radial Diffusion; Antigen is placed on the surrounding of the well

A

to determine if the antigens share identical epitope

23
Q

Double Radial Immunodiffusion:

Serological Identity

A

Smooth arc

24
Q

Double Radial Immunodiffusion:

Nonidentical

A

Crossed Lines

25
Q

Double Radial Immunodiffusion:

Partial Identity

A

Spur

26
Q

Measurement of Precipitation by Electrophoresis (3)

A
  1. Rocket Immunoelectrophoresis /One Dimension Electroimmunodiffusion
  2. Immunoelectrophoresis
  3. Immunofixation Electrophoresis
27
Q

RID + Electrophoresis
Antibody is uniformedly placed in the the gel
Antigen is placed in wells

A

Rocket Immunoelectrophoresis

28
Q

Rocket Immunoelectrophoresis

Positive Result

A

Precipitin Rockets

29
Q

Serum (containing antigens) is electrophoresed

Antibody is placed in TROUGH

Replaced by Immunofixation

A

Immunoelectrophoresis

30
Q

Serum (containing antigens) is electrophoresed

Antibody is overlaid directly to the Gel’s surface

A

Immunofixation Electrophoresis

31
Q

Immunofixation Electrophoresis (+)

A

Precipitin Bands

SEROLOGY (17 decks)

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