Fluorescent Immunoassay

Question 1

Fluorescent immunoassay uses ___ to label

Answer 1

Fluorophore / Fluorochrome

Question 2

Fluorophore / Fluorochrome that are used in Fluorescence Immunoassay

Answer 2

Fluorescein Isothiocyanate (FITC)
Tetramethyl Rhodamine Isothiocyanate (TRIC)
Phycoerythrin
Europium (B-napthyl trifluoroacetone)
Lucifer Yellow VS

Question 3

Fluorescence is measured using

Answer 3

Fluorometer
Fluorescence Microscope
Flow Cytometer
SpectroFluorometer

Question 4

Light Source used in measuring fluorescence

Answer 4

UV Light

Question 5

UV light emits ___ wavelength with _____ energy

Answer 5

LONGER wavelength
LOWER energy

Question 6

Fluorescence is _______ specific and sensitive

Answer 6

extremely

Question 7

Disadvantage of using Fluorescence Immunoassay (2)

Answer 7

Autofluorescence
Quenching

Question 8

Unknown antigen is placed on the slide and added with Fluorescence labeled antibody and then viewed under Fluorescence microscope

Answer 8

Direct Immunofluorescent

Question 9

Used same principle with Indirect ELISA; most commonly used

Answer 9

Indirect Immunofluorescent

Question 10

Blocking test by which the antigen is first exposed with unlabeled antibody, then to labeled antibody, and washed.

If unlabeled antibody and labeled antibody is homologous into the antigen it must have _______________

Answer 10

Inhibition Immunofluorescent Assay

NO FLUORESCENCE

Question 11

Based on the CHANGE IN POLARIZARION OF FLUORESCENT TAG emitted from labeled molecule when it is bound by antibody

Degree of polarization is _______ proportional to analyte concentration.

Answer 11

Fluorescence Polarization Immunoassay (FPIA)

INVERSELY proportional