Agglutination

Question 1

Process by which PARTICULATE ANTIGENS such as cells aggregate to form large complexes when exposed to specific antibody

Answer 1

Agglutination

Question 2

Antigen-antibody conbined through SINGLE antigenic determinant; rapid and reversible

Answer 2

Sensitization

Question 3

Sum of interactions between antibody and MULTIPLE ANTIGENIC DETERMINANT; “Crosslink” that forms visible aggregate

Answer 3

Lattice Formation

Question 4

Agglutination Reactions involved in:

Hemagglutination
Widal’s Test

Answer 4

Direct Agglutination

Question 5

Agglutination by which the antigen is found naturally on the SURFACE of particulate

Answer 5

Direct Agglutination

Question 6

Fourfold increase in Widal’s test is clinically significant to

Answer 6

Typhoid Fever

Question 7

Artificially placing ANTIGEN on the carrier particulate (charcoal, bentonite, RBC, Latex, Gel, Silicates)

Agglutination only occurs in the present of ANTIBODY

Answer 7

Indirect / Passive Agglutination

Question 8

Artificially placing ANTIBODY on the carrier particulate (charcoal, bentonite, RBC, Latex, Gel, Silicates)

Agglutination only occurs in the present of ANTIGEN

Answer 8

Reverse Passive Agglutination

Question 9

Competition of particulate and soluble antigens

Positive Result: Lack of Inhibition

Answer 9

Agglutination Inhibition

Question 10

HCG tests, Hemagglutination Inhibition

Answer 10

Agglutination Inhibition

Question 11

Uses bacteria for the attachment of Antibody

Answer 11

Coagglutination

Question 12

Portion of the Bacteria used in Coagglutination

Answer 12

Protein A - S. aureus

Question 13

Detection of non-agglutinating antibody by coupling with secondary antibody

Answer 13

Anti-globulin mediated agglutination

Question 14

Antiglobulin Mediated Test

Spx: Red Cell
In VIVO sensitization
Used in HD, AIHA, DHA, HTR

Answer 14

Direct Antiglobulin Test

Question 15

Antiglobulin Mediated Test:

Spx: Serum
In VITRO sensitization

Answer 15

Indirect Antiglobulin Test

Question 16

Antiglobulin Mediated Agglutination used in

• Crossmatching
• Antibody Determination and Identification
• Red cell antigen Phenotyping (Red cell)

Answer 16

Indirect AHG

Question 17

AHG Reagent

uses Anti-IgG and Anti-C3d
retrieved from Rabbit

Answer 17

Polyspecific AHG

Question 18

AHG Reagent

uses Anti-IgG OR Anti-C3d
retrieved from mice

Answer 18

Monospecific AHG

Question 19

Sensitized with IgG used to validate a negative reaction

Answer 19

Group O check cells

Question 20

After addition of Check O cells, agglutination makes

Answer 20

1. AHG was added
2. AHG was not neutralized

Question 21

In Group O check cells, this exhibit an invalidate result

Answer 21

Lack of Agglutination

Question 22

Quantitative Agglutination Reactions

Answer 22

Sol Particle Immunoassay (SPIA)
Dispersed Dye Immunoassay (DIA)
Immunoassay Particle Count (IMPACT)

Question 23

It is instrumentation used for measurement of number of residual non-agglutinating antibody by using LASER BEAM

Answer 23

PACIA
Particle Counting Immunoassay

Question 24

IgG that cannot bind with Protein A

Answer 24

IgG3

Question 25

Medication that causes (+) DAT:

Drug adsorption

Answer 25

Penicillin

Question 26

Medication that causes (+) DAT:

Membrane modification

Answer 26

Cephalosporin

Question 27

Medication that causes (+) DAT:

Immune-complex formation

Answer 27

PaRa Sa Immune-Complex

Phenacetin
Rifampin
Stibufen

Question 28

Medication that causes (+) DAT:

Autoantibody Formation

Answer 28

MaM

Methyldopa
Mefanamic acid

Question 29

hCG injection (Pregnyl)
Chorioepithelioma
Hydatidiform mole
Excessive ingestion of Aspirin

Answer 29

False Positive hCG Test