Proteins part 5

Question 1

• Causes y-globulins to migrate toward the cathode even though they are slightly (-) charged
• Due to electrical charge on support medium

Answer 1

ELECTROENDOSMOSIS

Question 2

• Relative concentration of each bond determined by _____________?

Answer 2

densitometry

Question 3

Plasma samples result in fibrinogen peak between gamma and beta fractions

Answer 3

Specimen collection and handling

Question 4

• Due to IgA (in serum); Cirrhosis
• Seen in patients with liver cirrhosis cause by increase in IgA

Answer 4

 Beta-gamma bridging effect:

Question 5

• Monoclonal gammopathy (Multiple myeloma)

Answer 5

 Monoclonal band (gamma-globulin);

Question 6

• Chronic inflammation

Answer 6

 Poluclonal band

Question 7

• nephrotic syndrome

Answer 7

 Increase in a-2-macroglobulin

Question 8

deficiency in a-a-antitrypsin; Juvenile cirrhosis

Answer 8

 a-1-globulin flat curve:

Question 9

• Seen in patients with Monoclonal gammopathy

Answer 9

Gamma spike:

Question 10

• Seen in patients with emphysema

Answer 10

• A-1-antitrypsin

Question 11

• Increased beta region, use plasma instead of serum.

Answer 11

• A-1-antitrypsin

Question 12

What is the reference range for total protein and Albumin?

Answer 12

• Total protein: 6.5 – 8.3g/dL
• Albumin: 3.5 – 5.5g/dL