LIVER FUNCTION TEST part 3

Question 1

What is the average daily bilirubin production?

Answer 1

250-300mg

Question 2

85% of bilirubin is derived from this.

Answer 2

Hgb

Question 3

15% of __________________ destroyed in the BM

Answer 3

Erythrocyte precursors

Question 4

What are the protein involved in the catabolism of other heme?

Answer 4

Myoglobin, Cytochromes & Peroxidases

Question 5

What ate the forms of Bilirubin?

Answer 5

Unconjugated
Conjugated
Delta

Question 6

bound by albumin & transported to the liver; water - insoluble

Answer 6

Unconjugated

Question 7

orange-yellow pigment derived from the hemoglobin degradation, it is extracted and bio transforming in the liver and excreted in the bile and urine

Answer 7

Unconjugated

Question 8

It is the main transporter of Unconjugated Bilirubin

Answer 8

Albumin

Question 9

It is a subtype of CB wherein it is none-polar, water insoluble and referred to as “indirect bilirubin” or hemobilirubin.

Answer 9

Beta 1

Question 10

What is the other name of B1?

Answer 10

“indirect bilirubin” or “hemobilirubin”

Question 11

formed from the reaction of UCB with glucuronic acid; polar and water soluble

Answer 11

Conjugated

Question 12

Conjugated Bilirubin is AKA as?

Answer 12

Bilirubin diglucuronide

Question 13

It is AKA ‘direct bilirubin”?

Answer 13

Beta 2

Question 14

conjugated bilirubin bound to Albumin

Answer 14

Delta

Question 15

Introduced the Diazo reaction for the detection of Bilirubin using urine

Answer 15

Ehrlich (1883)

Question 16

In the Diazo reaction, what acid is reacted with bilirubin?

Answer 16

diazotized sulfanilic acid

Question 17

applied the Diazo reaction in serum in the presence of an accelerator (solubilizer)

Answer 17

Van den Bergh & Snapper (1913)

Question 18

discovered the polar (direct reading) &non-polar (indirect reading) types of bilirubin in serum

Answer 18

Van den Bergh & Muller (1916)

Question 19

50% CH3OH as the non-polar solvent; first useful quantitative method for bilirubin

Answer 19

Evelyn & Malloy (1937)

Question 20

used the Diazo reaction with caffeine-benzoate-acetate as an accelerator

Answer 20

Jendrassik & Grof (1938)

Question 21

Published a candidate reference method for Total Bilirubin: _____________________?

Answer 21

Modified Jendrassik-Grof

Question 22

Agencies for the methods used?

Answer 22

American Association for Clinical Chemistry (AACC)
National Bureau of Standards (NBS)

Question 23

 both have acceptable precision
 adapted by many automated instruments
 most frequently used methods to measure bilrubin

Answer 23

JENDRASSIK-GROF & EVELYN-MALLOY PROCEDURES

Question 24

What is the color of the diazo product in acid pH?

Answer 24

Red to reddish-purple color

Question 25

This terminates the diazo reaction

Answer 25

Ascorbic acid

Question 26

This serves or acts as a buffer in Jendrassik-Grof reaction.

Answer 26

Sodium acetate

Question 27

the addition of _____________ converts the original purple color into _______ which can be measured spectrometrically at ___________

Answer 27

strongly alkaline tartrate
blue
600 nm