LIVER FUNCTION TEST part 3 Flashcards
What is the average daily bilirubin production?
250-300mg
85% of bilirubin is derived from this.
Hgb
15% of __________________ destroyed in the BM
Erythrocyte precursors
What are the protein involved in the catabolism of other heme?
Myoglobin, Cytochromes & Peroxidases
What ate the forms of Bilirubin?
Unconjugated
Conjugated
Delta
bound by albumin & transported to the liver; water - insoluble
Unconjugated
orange-yellow pigment derived from the hemoglobin degradation, it is extracted and bio transforming in the liver and excreted in the bile and urine
Unconjugated
It is the main transporter of Unconjugated Bilirubin
Albumin
It is a subtype of CB wherein it is none-polar, water insoluble and referred to as “indirect bilirubin” or hemobilirubin.
Beta 1
What is the other name of B1?
“indirect bilirubin” or “hemobilirubin”
formed from the reaction of UCB with glucuronic acid; polar and water soluble
Conjugated
Conjugated Bilirubin is AKA as?
Bilirubin diglucuronide
It is AKA ‘direct bilirubin”?
Beta 2
conjugated bilirubin bound to Albumin
Delta
Introduced the Diazo reaction for the detection of Bilirubin using urine
Ehrlich (1883)
In the Diazo reaction, what acid is reacted with bilirubin?
diazotized sulfanilic acid
applied the Diazo reaction in serum in the presence of an accelerator (solubilizer)
Van den Bergh & Snapper (1913)
discovered the polar (direct reading) &non-polar (indirect reading) types of bilirubin in serum
Van den Bergh & Muller (1916)
50% CH3OH as the non-polar solvent; first useful quantitative method for bilirubin
Evelyn & Malloy (1937)
used the Diazo reaction with caffeine-benzoate-acetate as an accelerator
Jendrassik & Grof (1938)
Published a candidate reference method for Total Bilirubin: _____________________?
Modified Jendrassik-Grof
Agencies for the methods used?
American Association for Clinical Chemistry (AACC)
National Bureau of Standards (NBS)
both have acceptable precision
adapted by many automated instruments
most frequently used methods to measure bilrubin
JENDRASSIK-GROF & EVELYN-MALLOY PROCEDURES
What is the color of the diazo product in acid pH?
Red to reddish-purple color
This terminates the diazo reaction
Ascorbic acid
This serves or acts as a buffer in Jendrassik-Grof reaction.
Sodium acetate
the addition of _____________ converts the original purple color into _______ which can be measured spectrometrically at ___________
strongly alkaline tartrate
blue
600 nm
