KIDNEY FUNCTION TEST part 5 Flashcards
What is the reagent used in Phophotungstic Acid (PTA) or Caraway method?
Phophotungstic Acid (PTA)
This substance is used to increase color and inhibit fading but considered to be hazardous?
Sodium Cyanide
What could be the alternative of Sodium cyanide?
Sodium Carbonate
If Sodium Carbonate is used, it is adjusted to what pH?
10.0 - 10.4
What is the positive result of PTA?
Tungsten blue
Tungsten blue is measured at what wavelength?
660 - 700 nm
What substances can lead to falsely elevated results?
Aspirin
Acetaminophen
Caffeine
Theophylline
This method determines the decrease in the UA concentration.
Blaunch and Koch (UV test with Uricase)
What happens in the first step of Blaunch and Koch (UV test with Uricase)?
Uricase converts UA to Allantoin and Hydrogen peroxide
What happens in the second step of Blaunch and Koch (UV test with Uricase)?
UA absorbs light at 285 nm.
What is the mode of reaction of Blaunch and Koch (UV test with Uricase)?
Decreasing
What is the relationship of decreased in absorbance in UV method to concentration of the UA?
Proportional
DHBS stands for?
Dihydroxy benzene sulfonate
This makes the product a yellow lutidine
Acetylacetone
This makes the product a tinder pink compound.
PAP
What is the color of copper neocuproine complex?
Yellow - orange
These uses phosphotungstic acid chromophores.
Bittner method
Two samples measurements is needed, ehat happens in the first measurement and second?
First: treated with uricase to destroy UA
Second: Uricase is absent
Cuprous ions combined with neocuproine will yield a product of?
Copper neocuproine complex
When Ferrous ions is reacted with TPTZ, what would be the product?
TPTZ method by Morin
In the coupled enzymatic method, uricase is coupled with?
Peroxidase
What substances can a false decrease result in BUA?
Reducing substances
What is measured in Coupled enzymatic method?
Color change
What are the 2 formation under Hyperurecemia?
Increased Formation
Decreased formation
Under decreased Excreation of Hyperurecemia, what is the drug therapy and poisons for secondary?
Salicylate
Heavy metal