LIPIDS AND LIPOPROTEINS part 3

Question 1

What are the interferences of Enzymatic method?

Answer 1

Elevated levels of ascorbic acid; hemoglobin and bilirubin

Question 2

What value causes the bilirubin to decrease cholesterol?

Answer 2

5 mg/dL

Question 3

If Bilirubin exceeds 5mg/dL it decreases cholesterol by how many percent?

Answer 3

5-15%

Question 4

What enzyme acted on cholesterol ester and water to form cholesterol and fatty acid?

Answer 4

Cholesterol esterase

Question 5

What enzyme acted on Cholesterol and oxygen to produce Cholest-4-en-one and hydrogen peroxide?

Answer 5

Cholesterol Oxidase

Question 6

What enzyme acted on hydrogen peroxide and 4-aminanotipyrine to produce quinoneimine dye?

Answer 6

Peroxidase

Question 7

4-anminoantipyrine is replaceable by ___________ on the third step.

Answer 7

Methanol

Question 8

In the presence of peroxidase, what happens to methanol?

Answer 8

It is oxidized into formaldehyde

Question 9

It is used in detecting acetylaceton

Answer 9

Formaldehyde

Question 10

In other methods, Cholest-4-en-3-one or cholesterol is measured at?

Answer 10

240nm

Question 11

The absorbance of Quinoneimine dye is measured at?

Answer 11

500nm

Question 12

4-aminoantipyrine is AKA as?

Answer 12

4-aminophenazone

Question 13

this uses cholesterol ester, hydrolase, and cholesterol oxidase

Answer 13

Oxygen consumption

Question 14

What causes the formation of water and oxygen under oxygen consumption?

Answer 14

Hydrogen peroxide is mixed with peroxidase

Question 15

this measures the oxygen released after the decomposition of hydrogen peroxide

Answer 15

Oxygen electrode

Question 16

It is not easily automated unlike others, it generally requires a lot of cholesterol oxidase

Answer 16

Oxygen consumption under cholesterol

Question 17

Oxygen consumption under cholesterol requires a lot of?

Answer 17

Cholesterol oxidase

Question 18

How many molecules of FA and Glycerol do TGC contain?

Answer 18

3
1

Question 19

The molecules of FA and Glycerol is held by what bond?

Answer 19

Ester bond

Question 20

What is the characteristic of TGC?

Answer 20

Hydrophobic and water insoluble

Question 21

main storage formof lipid

Answer 21

TGC

Question 22

Where is TCG found?

Answer 22

Adipose tissues

Question 23

Where is TCG from?

Answer 23

Plant sources rich in unsaturated FA

Question 24

TCG from animal sources contains?

Answer 24

Saturated FA which are solid at room temp

Question 25

TGC does not contain?

Answer 25

Charged or hydrophilic groups

Question 26

What compactly store long carbon chains (FA) for energy that can be used during fastong states between meals?

Answer 26

Bod

Question 27

TGC serves as an?

Answer 27

Energy source
Insulation
shock absorber
Integral part of cell membrane

Question 28

The breakdown of TAG is facilitated by?

Answer 28

LPL (Lipoprotein Lipase)
Epinephrine
Cortisol

Question 29

How many hours is the fasting requirements of TGC?

Answer 29

10-12hrs

Question 30

< 150 mg/dL

Answer 30

Normal

Question 31

150-199 mg/dL

Answer 31

Borderline high

Question 32

200-499 mg/dL

Answer 32

High TAG

Question 33

> 500

Answer 33

Very high TAG (acute and recurrent pancreatitis)

Question 34

What do TCG evaluates and measure?

Answer 34

Suspected atherosclerosis
body’s ability to metabolize fat

Question 35

What value of TGC is at risk for coronary artery disease?

Answer 35

Less than or equal to 200

Question 36

What is the most important lipids in the management of coronary artery disease?

Answer 36

TCG and cholesterol

Question 37

What causes the decrease of TAG level by 50%

Answer 37

Postural changes

Question 38

What are the interferences in the methods for TGC?

Answer 38

Ascorbic acid
Bilirubin
Hemolysis

Question 39

TAG level increases at _______ mg/dL/year between 45 to 65 years. old

Answer 39

2