LIPIDS AND LIPOPROTEINS part 3 Flashcards
What are the interferences of Enzymatic method?
Elevated levels of ascorbic acid; hemoglobin and bilirubin
What value causes the bilirubin to decrease cholesterol?
5 mg/dL
If Bilirubin exceeds 5mg/dL it decreases cholesterol by how many percent?
5-15%
What enzyme acted on cholesterol ester and water to form cholesterol and fatty acid?
Cholesterol esterase
What enzyme acted on Cholesterol and oxygen to produce Cholest-4-en-one and hydrogen peroxide?
Cholesterol Oxidase
What enzyme acted on hydrogen peroxide and 4-aminanotipyrine to produce quinoneimine dye?
Peroxidase
4-anminoantipyrine is replaceable by ___________ on the third step.
Methanol
In the presence of peroxidase, what happens to methanol?
It is oxidized into formaldehyde
It is used in detecting acetylaceton
Formaldehyde
In other methods, Cholest-4-en-3-one or cholesterol is measured at?
240nm
The absorbance of Quinoneimine dye is measured at?
500nm
4-aminoantipyrine is AKA as?
4-aminophenazone
this uses cholesterol ester, hydrolase, and cholesterol oxidase
Oxygen consumption
What causes the formation of water and oxygen under oxygen consumption?
Hydrogen peroxide is mixed with peroxidase
this measures the oxygen released after the decomposition of hydrogen peroxide
Oxygen electrode
It is not easily automated unlike others, it generally requires a lot of cholesterol oxidase
Oxygen consumption under cholesterol
Oxygen consumption under cholesterol requires a lot of?
Cholesterol oxidase
How many molecules of FA and Glycerol do TGC contain?
3
1
The molecules of FA and Glycerol is held by what bond?
Ester bond
What is the characteristic of TGC?
Hydrophobic and water insoluble
main storage formof lipid
TGC
Where is TCG found?
Adipose tissues
Where is TCG from?
Plant sources rich in unsaturated FA
TCG from animal sources contains?
Saturated FA which are solid at room temp
TGC does not contain?
Charged or hydrophilic groups
What compactly store long carbon chains (FA) for energy that can be used during fastong states between meals?
Bod
TGC serves as an?
Energy source
Insulation
shock absorber
Integral part of cell membrane
The breakdown of TAG is facilitated by?
LPL (Lipoprotein Lipase)
Epinephrine
Cortisol
How many hours is the fasting requirements of TGC?
10-12hrs
< 150 mg/dL
Normal
150-199 mg/dL
Borderline high
200-499 mg/dL
High TAG
> 500
Very high TAG (acute and recurrent pancreatitis)
What do TCG evaluates and measure?
Suspected atherosclerosis
body’s ability to metabolize fat
What value of TGC is at risk for coronary artery disease?
Less than or equal to 200
What is the most important lipids in the management of coronary artery disease?
TCG and cholesterol
What causes the decrease of TAG level by 50%
Postural changes
What are the interferences in the methods for TGC?
Ascorbic acid
Bilirubin
Hemolysis
TAG level increases at _______ mg/dL/year between 45 to 65 years. old
2
