LIVER FUNCTION TEST part 4 Flashcards
uses Diazotized sulfanilic acid for Bilirubin used in serum
Van Den Berg Reaction
Van Den Berg Reaction uses this acid in serum.
Diazotized sulfanilic acid
the diazotized sulfanilic acid is formed by reacting sulfanilic acid with _________________
sodium nitrate and hydrochloric acid
This is often subjective and the test involves diluting serum with saline until it visually
matches the color of a 0.01% potassium dichromate solution
Icterus Index
Icterus Index test involves diluting serum with __________ until it visually matches the color of a ____________________
saline
0.01% potassium dichromate solution
What are the interferences in Icterus Index?
Carotene
Xanthophyll
Hemoglobin
In DIRECT SPECTROPHOTOMETRIC METHOD, what is the wavelength at which bilirubin in serum is measured?
455nm
the absorbance of hemoglobin is 455 nm which can be corrected by subtracting the absorbance at ____________
575nm
Problem causing jaundice occurs before liver metabolism
Pre-hepatic Jaundice
What caused the pre-hepatic Jaundice?
Increased amount of bilirubin being presented in the liver
Unconjugated hyperbilirubinemia
PRE-HEPATIC JAUNDICE
Intrinsic liver disease
HEPATIC JAUNDICE
In Gilbert’s Syndrome, this is reduced in the expression of gene.
UGT1A1
intermittent hyperbilirubinemia
inherited bilirubin derangement
Reduced conjugating capability of the liver
Gilbert’s Syndrome
has a defective hepatic uptake of bilirubin
Gilbert’s Syndrome
has a defective conjugation of bilirubin due to the deficiency of the enzyme UDPGT
Crigler-Najjar Syndrome
- Chronic unconjugated hyperbilirubinemia
- Molecular defect w/in the gene involved with bilirubin conjugation
- Rare & more serious disorde
Crigler-Najjar Syndrome
total UDPGT deficiency
complete absence of enzymatic bilirubin conjugation
Type I Crigler-Najjar Syndrome
partial UDPGT deficiency
the mutation causing a severe deficiency of the enzyme for Conjugation
Type II Crigler-Najjar Syndrome
defective hepatic excretion of bilirubin
Dubin-Johnson Syndrome
What caused the Dubin-Johnson Syndrome?
deficiency of the canalicular transporter protein (MDR2/CMOAT)
defective hepatic excretion of bilirubin; has normal gall bladder function
Rotor Syndrome
What causes the Rotor Syndrome?
reduction in the concentration or activity of intracellular binding proteins.
TRUE OR FALSE:
Liver biopsy does NOT show dark pigmented granules
TRUE
it has a presence of circulating antibody against UDPGT
Lucey-Driscoll Syndrome
deposition of bilirubin in the nuclei of brain & nerve
cells-cell damage & death of newborn
Kernicterus
Usually results from biliary obstructive disease (e.g. gallstones, tumors)
Prevents the flow of conjugated bilirubin into the bile
canaliculi
Clay-colored stool
POST-HEPATIC JAUNDICE
What happens when bilirubin is exposed to light?
Photo-oxidize
conjugated bilirubin that is covalently bound to
albumin
Delta Bilirubin
the ______________ enhances or accelerates the unconjugated bilirubin reaction with diazotized sulfanilic acid
accelerator agents
