KIDNEY FUNCTION TEST part 3 Flashcards
What are the 3 categories of Azotemia?
Pre-renal Azotemia
Renal Azotemia
Post-renal Azotemia
This category reduces blood flow to the kidneys, increase protein catabolism or degradation and increase urea w/o an increase in creatinine.
Pre-renal Azotemia
What happens if less urea is excreted?
More urea is retained in the blood
Under Renal Azotemia, what is the problem?
There is a defect in the glomerular filtration rate and there are lesions along renal parenchyma.
What happens in the Post-renal azotemia?
Obstruction of Urine flow
What is the normal BUN:CREATININE RATIO?
10-20:1
What is the other name of CREATININE?
Anhydride of creatine
How is creatinine produced?
- Creatine loses water
- Creatine phosphate loses phosphoric acid (dephosphorylation)
What is the end product of muscle metabolism?
Creatinine
What can be used to assess the completeness of a 24-hr urine specimen since this is excreted at a constant rate?
Creatinine
The principal waste product of muscle metabolism is derived from?
Creatine
This is used as an accurate monitor of kidney function.
Creatinine
How is the formation of Creatine?
Transamination of Arginine and Lysine forming guanidinoacetic acid. As well as the methylation of guanidinoacetic acid.
What is the muscles’ energy source?
Creatine Phosphate
The Renal handling of Creatinine is via?
Glomerular filtration
What is done to obtain the value of creatinine?
apply Jaffe reaction to trichloroacetic acid filtrate of serum
What would be the product if Creatinine is reacted with Alkaline picrate?
Creatine picrate (red/yellow)
What is the composition of Alkaline picrate?
Picric acid + sodium hydroxide
What is the product measured under colorimetric: Jaffe’s reaction?
Red-orange complex known as Janovsky complex
At what wavelength is Janovsky complex measured?
490 - 505 nm
What is the preferred than the end point Jaffe reaction?
Kinetic Jaffe reaction
In this reaction, most interfering substances require longer time before contributing significantly to the absorbance which happens in the end point.
Kinetic Jaffe Reaction
This uses hydrated aluminum silicate to absorb
interfering substances.
Lloyd’s reagent / Fuller’s earth
What is the absorbent that Lloyd’s reagent use?
Sodium Aluminum silicate
What is the absorbent that Fuller’s earth use?
Aluminum magnesium silicate
