Protein Synthesis Flashcards
Protein is synthesized from the ___ terminal to the ____ terminal
amino; carboxyl
Each ribosome contains a ___ site and an ___ site
P (peptidyl); A (acceptor)
Processed mRNA enters the cytoplasm where it associates with a ___
ribosome
A ___ RNA molecule (bearing the amino acid coded for in the first codon) binds to this codon in the mRNA
transfer
Two AAs are joined by a ___ bond
peptide
___ factors help to terminate protein synthesis. For some proteins, membrane targeting is largely determined by the composition of the first twenty or so AAs in its amino terminal. This amino sequence where the protein will be targeted is termed the ___ ___
releasing; signal peptide
All tRNA molecules are folded into a ___ structure. This folding is accomplished by ____ bonding between complementary bases
cloverleaf; hydrogen
Every tRNA molecule studied possesses 4 distinctive regions. For the region listed, name its significance:
The 3’ end of the molecule
the AA binding site
note: terminates with the sequences C-C-A
Every tRNA molecule studied possesses 4 distinctive regions. For the region listed, name its significance:
The TC loop
this is where the tRNA binds to the ribosome
Every tRNA molecule studied possesses 4 distinctive regions. For the region listed, name its significance:
The D loop
this loop contains a high percentage of dihydrouridine residues (recognized by the enzyme tRNA synthetase)
Every tRNA molecule studied possesses 4 distinctive regions. For the region listed, name its significance: anticodon loop
this loop contains the anticodon which is complementary to the codon of the messenger RNA
What are the possible functions of the rare or minor bases present in tRNA?
a. to disallow base pairing at certain points in order to form the cloverleaf structure
b. to disallow unwanted base pairing with the mRNA
c. to protect the tRNA molecules from the action of ribonucleases
Although there are 20 AAs, the cells possess more than 20 types of tRNAs. Why?
there are several codons which specify a given AA
There are six codons for serine and several tRNA which recognize the various codons for serine (tRNAser). These tRNAs are called ____ because, although they recognize different codons, they all recognize serine
isoacceptors
Base pairing is antiparallel. tRNA runs __ –> ___ and mRNA runs ___ –> ___
3’ –> 5’; 5’ –> 3’
In regards to the wobble hypothesis, the third nucleotide of the codon is degenerate. Sometimes usual base pairing takes place between the ___ base of the codon and the ___ base of the anticodon
third; first
For the following anticodon nucleotides; give the possible base pairs it can make in the wobble position with the codon:
U
G
I (inosine)
U in anticodon recgonizes A or G in codon
G in anticodon recognizes C or U in codon
I in anticodon recognizes U, C, or A in codon
The inosine nucleotide is formed by ____ ____ after tRNA is formed on certain tRNAs
adenosine deaminase
Three dimensional (tertiary) structure of tRNA molecules has shown that their molecules are __-shpaed. The __ end of the molecule is at one end and the ____ loop is at the other end.
L; 3’; anticodon
Activation involves the coupling of an AA to its corresponding tRNA. These reactions are catalyzed by a group of enzymes termed what?
amino acyl tRNA synthetases
The ___ bond between the AA and the tRNA molecules is a high energy bond. The AA is bound to the 2’ or 3’ - ___ group of the ribose of the terminal adenylate residue of tRNA
ester; OH
Since the pyrophosphate formed during the reaction is hydrolyzed, the activation process requires the hydrolysis of two high energy ____ bonds. This is equivalent to the use of __ ATPs
phosphate; 2
The aminoacyl-tRNA synthetases are highly specific for both the AA and tRNA that they bind. It is imperative that they exhibit a high degree of ___, since it is the ____ of the tRNA molecule which is recognized at the ribosome
specificity; anticodon
The number of tRNA synthetases is ___.
20