practical

Question 1

what is serum

Answer 1

fluid portion of clotted blood; straw colored fluid from fibrin clot
water + electrolytes

Question 2

steps to collect serum sample

Answer 2

collect (avoid hemolysis), allow to clot at room temp for 20-30min, centrifuge 10 min at 2-3000 rpm, pippette off
take plasma out or RBCs will continue to metabolize things like glucose

Question 3

for clin chemistry machine use what kinds of blood samples?

Answer 3

serum or heparanized plasma

Question 4

RBC formula for MCV (and units)

Answer 4

pcv x 10 / rbc count (millions) = fl

Question 5

MCH formula and units

Answer 5

hgb x 10 / rbc in millions = pg

Question 6

mchc formula and units

Answer 6

hgb x 100 / pcv = g/dl

Question 7

units and normal canine estimated platlet count

Answer 7

7-10 /HPF

less than 5 or 3 suggestive of thrombocytopenia

Question 8

how estimated platlet count is done

Answer 8

blood smear, examine 10 random fields in oil, divide total by 10 to avg -> # per HPF

Question 9

how long serum can be refrigerated for

Answer 9

72 hr

Question 10

total protein units

Answer 10

g/dl