Lab Quiz

Question 1

Which color top is usually used for antibody studies in animals

Answer 1

red

Question 2

choice anticoagulant for hematology testing (CBCs), why

Answer 2

EDTA, doesnt interfere w staining

Question 3

choice anticoagulant for blood chemistries

Answer 3

heparin

Question 4

how to do WBC count

Answer 4

lyse RBCs w acetic acid (1:20), use hemocytometer, 10x (low) power, count WBCs in 4 big corner squares

Question 5

how to calculate WBC count from cells counted

Answer 5

cells counted x 50= wbc/ul

Question 6

how to do differential WBC count

Answer 6

perform blood smear, differential stain, look under oil immersion, use battlement method of counting 100 cells

Question 7

how to calculate differential WBC for each kind of WBC

Answer 7

cells counted (%) x WBC count= WBC/ul
(ex: .72 x 20,000 wbcs= /ul)

Question 8

how to do RBC count

Answer 8

dilute w physiologic saline solution (1:200), count 5 boxes in center square on 40x (high power)

Question 9

what refractometer is used for

Answer 9

Total protein or specific gravity

Question 10

what hemocytometer is used for

Answer 10

WBC count (not differential) and RBC count

Question 11

how to calculate RBC count from RBCs counted on hemocytometer

Answer 11

multiple by 100,000= RBCs/ ul

Question 12

troubleshooting for if smear is too short or small

Answer 12

larger drop, decrease angle, decrease speed

Question 13

troubleshooting for if smear extends to ends of slide

Answer 13

smaller drop, increase angle, increase speed

Question 14

troubleshooting for smear w waves or ridges

Answer 14

relax wrist (too much force), or increase speed

Question 15

troubleshooting for smear too thick

Answer 15

smaller drop, decrease angle, increase speed

Question 16

troubleshooting for smear too thin

Answer 16

larger drop, increase angle, decrease speed

Question 17

how long to centrifuge dog and ruminant pcv

Answer 17

dog=  5 min
ruminant= 10 min (both 10,000 RPM)