CBC (PCV and Diff white blood cell count)

Question 1

what is a CBC

Answer 1

Complete blood count- groups of tests to evaluate the circulating blood

Question 2

tests in a CBC

Answer 2

PCV, TP, WBC count, differential WBC count, RBC count, hemoglobin values, Indices (formulas)

Question 3

PCV, what it stands for, what it is also known as

Answer 3

Packed Cell volume, Hematocrit

Question 4

best test to determine RBC number or O2 carrying ability (is there enough RBCs?)

Answer 4

PCV/ hematocrit

Question 5

tubes used for PCV test

Answer 5

microhematocrit tubes

Question 6

difference between blue and red microhematocrit tubes

Answer 6

red= heparinized (has anticoagulant) take blood directly from animal
blue= non-heparinized (no anticoag.) take blood from whole blood sample

Question 7

how fast and how long to centrifuge d/c blood and ruminant blood

Answer 7

d/c= 10,000 rpm, 5 min
ruminants== 10,000 rpm, 10min

Question 8

the clear portion of PCV tube is what

Answer 8

plasma

Question 9

what the thin white portion of a PCV tube is

Answer 9

the buffy coat (WBCs and platelets)

Question 10

read hematocrit tubes at; top of rbcs or top of buffy coat

Answer 10

top of rbcs

Question 11

normal dog PCV reading (average and range)

Answer 11

avg= 45% range= 37%-55%

Question 12

low pcv % indicates

Answer 12

anemia

Question 13

high pcv % indicates

Answer 13

dehydration

Question 14

what pink plasma indicates

Answer 14

hemolysis

Question 15

what yellow plasma is called

Answer 15

icterus (could be # of diseases)

Question 16

what white plasma indicates

Answer 16

Lipemia (fat in blood) indicates that the animal ate recently

Question 17

what a blood smear is used for

Answer 17

differential WBC count

Question 18

how romanowsky staining works

Answer 18

based on pH of components

Question 19

3 solutions in dif-quick process

Answer 19

fixative(green), eosin(red/orange), Methylene blue

Question 20

purpose of fixative (green) solution in dif-quick procedure

Answer 20

preserve cells on slide, preps for stain, helps take up stain

Question 21

for each solution in a dif-quick stain, how long to dip and how many times

Answer 21

5 times, 1 second dips

Question 22

which stain is basic, which is acidic

Answer 22

eosin= acidic

methylene blue= basic

Question 23

basic components of cells, what stain they attract?

Answer 23

hemoglobin, cytoplasm, granuals in some WBCs
attract eosin (opposite)

Question 24

acidic components of cells, what stain they attract?

Answer 24

nucleus (DNA), some WBC granuals

attract methylene blue (opposite)

Question 25

define basophilic in terms of what color it stains

Answer 25

stains blue (takes up basic dye, is an acidic component)

Question 26

define acidophilic/eosinophilic in terms of what color it stains

Answer 26

stains pink/orange (takes up acidic dye, is a basic component)

Question 27

what color a neutrophilic component stains

Answer 27

pink+blue/grey

Question 28

how new methylene blue staining works

Answer 28

blood mixed w methylene blue, smeared

Question 29

What is new methylene blue staining used for

Answer 29

Quick staining and screening