Pneumonia, TB, C. Diff, and Influenza Flashcards
What are important acid fast organisms
TB
Leprosy
Opportunistic wound infections
Can acid fast bacteria grow inside macrophages?
Yes
What other organisms get dyed with acid fast stain?
Norcardia
Skin and soft tissue infections associated with fish tanks
Mycobacterium marinum
Rapid grower and often a water contaminant. It is part of a group of environmental mycobacteria and is found in water, soil, and dust. It has been known to contaminate medications and products, including medical devices. It causes chronic lung disease, disseminated cutaneous disease in immunocompromised patients, and post traumatic wound infections.
Mycobacterium abcessus
Rapid grower, often found in water and sewage and will cause occasional opportunistic infections
Mycobacterium chelonae
A slow grower that is a water contaminant and may cause disease in patients with severely impaired cellular immunity
Mycobacterium kansasii
Hansen’s disease, it can affect the nerves, skin, eyes, and lining of the nose. It may take up to 20 years to develop signs of infection
Mycobacterium leprae
Rapid grower or slow grower? Mycobacterium abcessus
Rapid
Rapid grower or slow grower? Mycobacterium chelonae
Rapid
Rapid grower or slow grower? Mycobacterium kansasii
Slow
Rapid grower or slow grower? Mycobacterium chimaera
Slow
Rapid grower or slow grower? Mycobacterium fortuitum
Rapid
Rapid grower or slow grower? Mycobacterium avium complex
Slow
Rapid grower or slow grower? Mycobacterium mucogenicum
Rapid
C. Diff basics
Gram positive, spore forming rod
anaerobic
What are the main toxins of C. Diff
Toxin A
Toxin B
Binary Toxin
Describe Toxin A in C Diff
entertoxin and cytotoxin
Describe toxin B in C. Diff
cytotoxin
What isolate is binary toxin primarily found in?
B1/NAP1/027 isolate
Threat level of C. Diff
Urgent (highest level)
What antibiotics are the highest risk for CDI?
third gen cephalosporins
Description of stool related to CDI
Soft, unformed stools to watery or mucoid
How many bowel movements a day for C. Diff
3-20
How common are fever and abdominal pain with C. Diff
about 25%
What non-culture labs may be indicative of C. Diff
High WBC (ex 25k)
Positive occult fecal blood test
Predominant method of dx testing utilized for ID of C. Diff?
NAAT (nucleic acid amplification test)
Gold standard for C. Diff? Issue?
culture
takes 7 days
common complications of C. Diff
Pseudomembranous colitis
Toxic megacolon
Ileus
Perforation
Percentage of patients that exhibit pseudomembranous colitis
50%
What does pseudomembranous colitis look like?
White/ yellow plaques that show in endoscopy
what is toxic megacolon?
Extremely enlarged colon+ abdominal distention
What is ileus
painful obstruction of the ileum or other part of the intestine
Concern for perforation resulting from C. Diff
fecal matter leaks out - peritonitis
Where is BI/NAP1/027 strain widespread?
Throughout the U.S. and Europe
Concerns with B1/NAP1/027
Higher mortality (6.7%)
Can cause outbreaks in hospitals
Highly resistant to antimicrobials
General treatment options for C. Diff
Vancomycin
Fidaxomicin
Fecal microbiota transplantation
When would fecal microbiota transplantation be considered?
Multiple recurrances
What are the concerns with hypochlorite solution in cleaning for C Diff
Odor
Respiratory irritation
skin irritation
Damage to surface
has not been evaluated sufficiently for efficacy
What cleaner to use for C. Diff
Bleach
Where do the highest incidences of TB occur?
Africa (363/100,000)
Asia (180/100,000)
When did TB rates rise in the US?
1985-1992
Reason TB has decreased so much from the early 90s until 2011
- DOT
- Expanded treatment regimens
- CDC infection control recommendations
TB with resistance to INH, Rifampin, any fluoroquinolone, and at least 1 injectable second line drug
XDR-TB (extensively drug resistant TB)
Basics of M. tuberculosis
aerobic, acid fast bacillus
transmission of M. tuberculosis
Mainly via inhalation
Have caused a variety of skin diseases including pulmonary, skin, and soft tissue infections, esp in immunocompromised individuals
Nontuberculosis mycobacteria
Size of TB
1-5 um
who generates airborne TB particles
Patients with pulmonary and laryngeal TB
what is the concern for TB and ventilation
Can remain suspended in the air for a very long time and travel through ventilation system to other areas of building
What happens when the infectious droplet is breathed in?
Goes to the macrophages, gets phagocytised by macrophages, survive in macrophages and produce local infection. May also disseminate
If TB disseminates, where does it spread to first?
The regional lymph node
What is the initial infection of TB like?
Some have mild symptoms
usually goes unrecognized
Is the individual TB patient infectious during the initial infection?
No, not unless active disease developes
When does specific immunity develop for TB?
10-12 weeks
What happens when specific immunity developes?
Further spread of the organism is prevented
What does PPD stand for?
Purified protein derivative
What does IGRA stand for?
Interferon gamma release assay
when do the PPD/ IGRA tests become positive after infection?
2-12 weeks
What is LTBI?
Latent TB Infection - asymptomatic stage of TB
What is TB in the spine?
Potts Disease
How long after initial infection do some people develop active TB?
Within 2 years
Why do people develop active TB?
Failure of immune system to control mycobacteria
What percentage of people develop active TB?
5%
What is the annual risk for people with latent TB to develop TB if they do not develop it within the first 2 years?
5-10 percent
What is the total lifetime risk for developing active TB after initial infection?
10-15 percent
Symptoms of primary TB infection
- asymptomatic* (most) or
- fever
-cough
-erythema nodosum
Type of skin inflammation that is located in part of the fatty layer of the skin
Erythema nodosum
Where does erythema nodosum typically occur?
Front of legs below the knees
presentation for erythema nodosum
Reddish, painful, tender lumps
What is the radiological presentation of primary TB infection?
-Gohn complex
-miliary pattern in progressive cases
- Central casieous necrosis
Describe central caseous necrosis
encased mycobacteria in the lymphocytes
Unique form of cell death in which the tissue maintains a cheese-like appearance and the dead tissue appears as soft and white proteinaceous dead cell mass
Caseous necrosis
Frist symptoms of post primary TB
-Productive Cough
-Fever (37-80%)
-Night sweats
What does chest radiology show for postprimary TB?
- infiltrates on the upper lobes or superior segments of lower lobes
- cavitation on chest xrays
How to differentiate TB cough from other resp coughs
Last >2-3 weeks and produces sputum
Spitting of blood that originated in the lungs
Hemoptysis
Later symptoms of TB
-productive cough
- hemoptysis
- weight loss
- chest pain
-anorexia
-malaise
-debilitation
How does TB present (chest imagining) in patients with TB with higher CD4 T-cell counts
Typical infiltrates of postprimary TB
How do patients with HIV and low CD4 counts typically present with pulmonary TB in chest imaging?
Atypical
Miliary pattern
Lower lobe infiltrates
Sometimes normal chest xrays
_____ cell is the masterpiece of the immune response in TB, while the ______ is the effector cell
CD4 cell….
Macrophage
What are the names of the acid-fast stains?
Kinyoun or Ziehl Neelsen
What other organisms may stain acid-fast under some conditions?
Norcardia
Rhodococcus
Legionella micdadei
What is the fastest that MTB can be cultured?
With newer broths, a few days
What tests are commonly used to is TB before a sputum specimen is AFB positive
NAAT test
Why are cultures required for TB?
test for antimicrobial sensitivity
When to do susceptibility testing
initial isolate on every patient
+ if patient remains culture positive after 3 months of treatment
Injection of purified protein derived from mycobacterial cell wall
Tuberculin Skin Test (TST)